Methods for in vitro functional analysis of iPSC derived cardiomyocytes — Special focus on analyzing the mechanical beating behavior
Autor: | Antti Ahola, Katriina Aalto-Setälä, Jari Hyttinen, Eeva Laurila |
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Přispěvatelé: | Tampere University, Institute of Biomedical Technology, Department of Electronics and Communications Engineering, Research group: Computational Biophysics and Imaging Group, School of Medicine, BioMediTech |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Time Factors Action Potentials Nanotechnology Video microscopy Cell lineage 03 medical and health sciences Heart Rate Basic research Screening method Humans Cell Lineage Myocytes Cardiac Safety toxicology Molecular Biology Cells Cultured Analysis method Cell Proliferation Microscopy Video Stem cell derived cardiomyocytes Disease mechanisms Cell Differentiation Cell Biology 217 Medical engineering Myocardial Contraction Induced pluripotent stem cells 030104 developmental biology Disease modeling Early phase Neuroscience Functional analysis (psychology) |
Popis: | A rapidly increasing number of papers describing novel iPSC models for cardiac diseases are being published. To be able to understand the disease mechanisms in more detail, we should also take the full advantage of the various methods for analyzing these cell models. The traditionally and commonly used electrophysiological analysis methods have been recently accompanied by novel approaches for analyzing the mechanical beatingbehavior of the cardiomyocytes. In this review, we provide first a concise overview on the methodology for cardiomyocyte functional analysis and then concentrate on the video microscopy, which provides a promise for a new faster yet reliable method for cardiomyocyte functional analysis. We also show how analysis conditions may affect the results. Development of the methodology not only serves the basic research on the disease models, but could also provide the much needed efficient early phase screening method for cardiac safety toxicology. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel. publishedVersion |
Databáze: | OpenAIRE |
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