Mitochondrial DNA copy number changes in human gliomas
| Autor: | Laura Hays, Bertrand C. Liang |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Mitochondrial DNA DNA Complementary Molecular Sequence Data Gene Dosage EcoRI Glial tumor Mitochondrion DNA Mitochondrial chemistry.chemical_compound Complementary DNA Gene duplication Tumor Cells Cultured Humans Genetics Base Sequence biology Brain Neoplasms Gene Amplification DNA Neoplasm Glioma Gene Expression Regulation Neoplastic Blotting Southern Oncology chemistry Suppression subtractive hybridization biology.protein DNA |
| Zdroj: | Cancer Letters. 105:167-173 |
| ISSN: | 0304-3835 |
| Popis: | Gene amplification has been found to be biologically important in cancer. We report a complementary DNA sequence obtained using a subtractive hybridization approach which is frequently and highly amplified in human gliomas. 39/45 (87%) glial tumor specimens (of pathologically low and high grade) revealed increases in copy number of this clone from 5- to 25- fold; erb-b amplification was found in 8/45 (18%). This clone revealed homology to non-continuous mitochondrial DNA positions 1679-1948 and 2017-2057, with the interspersed sequences deleted. A non-mitochondrial genomic addition of 15 bases at the 5' end of the clone and a 7 base insertion adjacent to position 1948 were also present. Evaluation of the entire mitochondrial genome in a subset of 11 tumors showed maximal amplification between mitochondrial positions 748 and 5882, and a lower degree of amplification elsewhere, with a recurrent deletion of a 1.2 kb EcoRI fragment noted in 5/11 (46%) tumors. The mitochondrial genome is frequently affected in human gliomas, and warrants further study to determine its role in glial malignancy. |
| Databáze: | OpenAIRE |
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