Inhibition of renal gluconeogenesis and phosphoenolpyruvate carboxykinase activity by 3-mercaptopicolinic acid: studies in rat, guinea pig, dog, rabbit, and man
| Autor: | Guy Lemieux, Patrick Vinay, André Gougoux, Malcolm Watford |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
medicine.medical_specialty
Kidney Cortex Guinea Pigs Biology Kidney Guinea pig Cytosol Dogs Species Specificity Internal medicine medicine Animals Humans Phosphoenolpyruvate carboxykinase activity Distribution (pharmacology) Picolinic Acids chemistry.chemical_classification Gluconeogenesis General Medicine Mitochondria Rats Kidney Tubules Endocrinology Enzyme chemistry Phosphoenolpyruvate Carboxykinase (GTP) Rabbits Phosphoenolpyruvate carboxykinase Intracellular |
| Zdroj: | Canadian Journal of Biochemistry. 58:440-445 |
| ISSN: | 0008-4018 |
| DOI: | 10.1139/o80-058 |
| Popis: | The inhibition of gluconeogenesis in isolated kidney tubules by 3-mercaptopicolinate, a specific inhibitor of the phosphoenolpyruvate carboxykinase reaction, is species specific. At any given concentration of the inhibitor, the degree of inhibition is in the order rat > guinea pig ≥ man > rabbit ≥ dog. The intracellular distribution of PEPCK activity (as measured in both the forward and backward directions) supports the conclusion that in the intact cell the cytosolic enzyme is preferentially inhibited. Analysis of the inhibition failed to demonstrate any significant differences between the enzymes, regardless of intracellular or animal source. All enzymes exhibited a similar Ki (2–10 μM) for 3-mercaptopicolinate when assayed in the forward direction. The observed preferential cytosolic inhibition could be due to either in situ differences in metallic ions concentration or a restriction in mitochondrial permeability for mercaptopicolinate. |
| Databáze: | OpenAIRE |
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