Antioxidant and anti-inflammatory potential, and chemical composition of fractions of ethanol extract of Annona muricata leaf
| Autor: | G. A. Olatunji, Idorenyin Ugochi Nwaehujor, O. A. Fabiyi, S. A. Akande |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
radical scavenging
concentration ABTS Antioxidant Chromatography Science (General) biology DPPH medicine.medical_treatment Ethyl acetate General Medicine biology.organism_classification inhibition Tyrosol chemistry.chemical_compound Phytol Q1-390 chemistry bioactivity medicine Annona muricata Chemical composition |
| Zdroj: | Ruhuna Journal of Science, Vol 11, Iss 2, Pp 131-142 (2020) |
| ISSN: | 2536-8400 |
| Popis: | Serious health challenges have been associated with inflammation which is a major cause of mortality in the world. This study evaluated the antioxidant, anti-inflammatory potential, and chemical compositions of fractions of ethanol extract of Annona muricata leaf. The leaves were dried at room temperature, blended and extracted in sequential with solvents of varying degree of polarities, i.e., n-hexane, ethyl acetate and ethanol. Ethanol extract was fractionated via solvent-solvent partitioning into five fractions, i.e., n-hexane fraction (F1), dichloromethane fraction (F2), dichloromethane/ methanol (1:1) fraction (F3), methanol fraction (F4), and ethanol fraction (F5). These fractions were examined for their in-vitro antioxidant activities on DPPH, ABTS and H2O2 while the anti-inflammatory activities were investigated using lipoxygenase inhibition, proteinase inhibition and membrane stabilization assays. The F4 being the most active fraction was further analyzed with GCMS to determine its chemical compositions. The results showed that F4 had the highest H2O2 scavenging activity at 10–100 µg/mL. The activity of F4 at 50 µg/mL was significantly higher (P |
| Databáze: | OpenAIRE |
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