Ontogeny of clock and KiSS-1 metastasis-suppressor (Kiss1) gene expression in the prepubertal mouse hypothalamus
| Autor: | Brendan J. Waddell, Peter J. Mark, Cassandra Yap, Jeremy Troy Smith |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Period (gene) Hypothalamus CLOCK Proteins Biology 03 medical and health sciences Mice Endocrinology Kisspeptin Sex Factors Internal medicine Gene expression Genetics medicine Animals Circadian rhythm Molecular Biology Regulation of gene expression Kisspeptins Suprachiasmatic nucleus ARNTL Transcription Factors Gene Expression Regulation Developmental Circadian Rhythm CLOCK Mice Inbred C57BL 030104 developmental biology Reproductive Medicine Nuclear Receptor Subfamily 1 Group D Member 1 Animal Science and Zoology Female Proestrus Anteroventral periventricular nucleus hormones hormone substitutes and hormone antagonists Developmental Biology Biotechnology Receptors Kisspeptin-1 |
| Zdroj: | Reproduction, fertility, and development. 29(10) |
| ISSN: | 1031-3613 |
| Popis: | Kisspeptin is crucial for the generation of the circadian-gated preovulatory gonadotrophin-releasing hormone (GnRH)–LH surge in female rodents, with expression in the anteroventral periventricular nucleus (AVPV) peaking in the late afternoon of pro-oestrus. Given kisspeptin expression is established before puberty, the aim of the present study was to investigate kisspeptin and clock gene rhythms during the neonatal period. Anterior and posterior hypothalami were collected from C57BL/6J mice on Postnatal Days (P) 5, 15 and 25, at six time points across 24 h, for analysis of gene expression by reverse transcription–quantitative polymerase chain reaction. Expression of aryl hydrocarbon receptor nuclear translocator-like gene (Bmal1) and nuclear receptor subfamily 1, group D, member 2 (Rev-erbα) in the anterior hypothalamus (containing the suprachiasmatic nucleus) was not rhythmic at P5 or P15, but Bmal1 expression exhibited rhythmicity in P25 females, whereas Rev-erbα expression was rhythmic in P25 males. KiSS-1 metastasis-suppressor (Kiss1) expression did not exhibit time-of-day variation in the anterior (containing the AVPV) or posterior (containing the arcuate nucleus) hypothalami in female and male mice at P5, P15 or P25. The data indicate that the kisspeptin circadian peak in expression observed in the AVPV of pro-oestrous females does not manifest at P5, P15 or P25, likely due to inadequate oestrogenic stimuli, as well as incomplete development of clock gene rhythmicity before puberty. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|