Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor
Autor: | Maria Glyavina, Gary L. Horowitz, Lev T. Perelman, Mark F. Coughlan, Le Qiu, Vladimir Turzhitsky, Irving Itzkan, Edward Vitkin, Yuri Zakharov, Umar Khan, Lei Zhang |
---|---|
Rok vydání: | 2021 |
Předmět: |
Susceptibility testing
biology Chemistry Metals and Alloys Light-Scattering Spectroscopy Condensed Matter Physics medicine.disease biology.organism_classification Rapid detection Article Surfaces Coatings and Films Electronic Optical and Magnetic Materials Microbiology Sepsis Materials Chemistry medicine Identification (biology) Electrical and Electronic Engineering Instrumentation Biosensor Bacteria Whole blood |
Zdroj: | Sens Actuators B Chem |
ISSN: | 0925-4005 |
Popis: | Bacterial infections are one of the major causes of death worldwide. The identification of a bacterial species that is the source of an infection generally takes a long time, and often exceeds the treatment window for seriously ill patients. Many of these deaths are preventable if the bacterial species can be identified quickly. Here we present an optical spectroscopic method for rapid detection and identification of bacteria directly from whole blood using a light scattering spectroscopy technique. This technique was originally developed to detect pre-cancerous changes in epithelial tissues, characterize changes in tissue on the cellular scale, and characterize biological structures comparable to or smaller than a single wavelength. We demonstrate here that not only can an inexpensive light scattering spectroscopy-based biosensor rapidly detect and identify four bacteria species in the blood, responsible for the majority of death causing infections, but that species-level identification can potentially be made based on approximately one thousand bacterial cells per milliliter of blood. Observing entire colonies or performing susceptibility testing is therefore not required. |
Databáze: | OpenAIRE |
Externí odkaz: |