Heterotypic Cell-Cell Interaction on Micropatterned Surfaces
| Autor: | Stefania Lamponi, Rolando Barbucci, Clara Di Canio |
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| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Materials science Surface Properties Scanning electron microscope 030232 urology & nephrology Biomedical Engineering co-cultures endothelial cells fibroblasts micropatterned surfaces hyaluronic acid Medicine (miscellaneous) Bioengineering Video microscopy Nanotechnology Cell Communication 030204 cardiovascular system & hematology Microscopy Atomic Force Cell morphology Biomaterials 03 medical and health sciences 0302 clinical medicine Cell–cell interaction Cell Adhesion Humans Hyaluronic Acid Cell Shape Cells Cultured Microscopy Video Tissue Scaffolds Atomic force microscopy Endothelial Cells General Medicine Adhesion Fibroblasts Coculture Techniques Microscopy Electron Scanning Biophysics Glass Adhesive |
| Zdroj: | The International Journal of Artificial Organs. 32:507-516 |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139880903200805 |
| Popis: | PurposeThe aim of this paper was to study the influence of chemical and topographical signals on cell behavior and to obtain a heterotypic cell-cell interaction on microstructured domains.MethodsThe polysaccharide hyaluronic acid (Hyal) was photoimmobilized on glass surfaces in order to obtain a pattern with squares and rectangles of different dimensions and chemistry. The microstructured surfaces were characterized by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The behavior of Human Coronary Artery Endothelial Cells (HCAEC) and human tumoral dermal fibroblasts (C54) was investigated on these micropatterned surfaces by adhesion studies. Moreover, heterotypic interaction among C54 and HCAEC adherent on patterned surfaces was evaluated by time-lapse video microscopy.ResultsSurface analysis revealed the presence of a pattern consisting of alternating glass and Hyal microstructures whose dimensions decreased from the center to the edge of the sample. Neither HCAEC nor C54 adhered to the immobilized Hyal but both adapted their shape to the different sizes of the glass squares and rectangles. The number of adherent cells depended on the dimensions of both the glass domains and the nuclei of the cells. Co-cultured C54 on HCAEC patterned surfaces showed a heterotypic cell-cell interaction in the same chemical and topographic domain.ConclusionsA heterotypic cell-cell interaction occurred in the same chemical and topographic micro-domains but in narrow areas only. Moreover, the number of cells adhering to the glass domains and cell morphology depended on the dimensions of both adhesive areas and cell nuclei. |
| Databáze: | OpenAIRE |
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