Dynamics of microRNAs in bull spermatozoa
| Autor: | Erdogan Memili, Elizabeth A. Crate, Mehmet Osman Atli, Abdullah Kaya, James F. Padbury, Alper Uzun, La Shonda Robertson, Aruna Govindaraju, Andy D. Perkins, Einko Topper |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system lcsh:QH471-489 media_common.quotation_subject Fertility Biology lcsh:Gynecology and obstetrics 03 medical and health sciences 0302 clinical medicine Endocrinology microRNA Gene expression medicine lcsh:Reproduction Animals lcsh:RG1-991 Gametogenesis reproductive and urinary physiology 030304 developmental biology media_common Oligonucleotide Array Sequence Analysis Genetics 0303 health sciences 030219 obstetrics & reproductive medicine urogenital system Reverse Transcriptase Polymerase Chain Reaction Research Mammalian Embryogenesis Obstetrics and Gynecology Phenotype Sperm Spermatozoa MicroRNAs medicine.anatomical_structure Reproductive Medicine Fertilization Embryonic development Gamete Cattle Uncompensatory fertility Developmental Biology |
| Zdroj: | Reproductive Biology and Endocrinology : RB&E Reproductive Biology and Endocrinology, Vol 10, Iss 1, p 82 (2012) |
| ISSN: | 1477-7827 |
| Popis: | Background MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions. Methods To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets. Results We demonstrated that an abundance of microRNAs were present in bovine spermatozoa, however, only seven were differentially expressed; hsa-aga-3155, -8197, -6727, -11796, -14189, -6125, -13659. The abundance of miRNAs in the spermatozoa and the differential expression in sperm from high vs. low fertility bulls suggests that the miRNAs possibly play important functions in the regulating mechanisms of bovine spermatozoa. Conclusion Identification of specific microRNAs expressed in spermatozoa of bulls with different fertility phenotypes will help better understand mammalian gametogenesis and early development. |
| Databáze: | OpenAIRE |
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