Immunohistochemical analysis of inflammatory infiltrate in aggressive and chronic periodontitis: a comparative study
| Autor: | Daniel Ferrari, Luciano Artese, Jamil Awad Shibli, Maciej Simon, Luciana A. Gouveia de Cardoso, Tatiana Onuma, Marcello Piccirilli, Adriano Piattelli, Marcelo Faveri, Vittoria Perrotti |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Cell type Pathology medicine.medical_specialty Plasma Cells Antigens Differentiation Myelomonocytic CD8-Positive T-Lymphocytes Statistics Nonparametric Immunoenzyme Techniques Young Adult Antigen Antigens CD Humans Cytotoxic T cell Medicine Aggressive periodontitis General Dentistry Aged Cell specific business.industry Macrophages Case-control study Middle Aged Antigens CD20 medicine.disease Chronic periodontitis Lymphocyte Subsets Aggressive Periodontitis Case-Control Studies Chronic Periodontitis Immunology Immunohistochemistry Female Inflammation Mediators business |
| Zdroj: | Clinical Oral Investigations. 15:233-240 |
| ISSN: | 1436-3771 1432-6981 |
| DOI: | 10.1007/s00784-009-0374-1 |
| Popis: | This immunohistochemical study evaluated the inflammatory infiltrate with its cluster differentiation markers (CD 4, CD 8, CD 20, and CD 68) in aggressive and chronic periodontitis gingival tissues in order to identify the specific cell distribution. Twenty-seven human gingival biopsies were obtained and analyzed. Fourteen patients were suffering from chronic periodontitis and six from aggressive periodontitis; seven patients with healthy gingiva were included as the control group. The specimens were immunohistochemically stained for anti-CD 4 (T helper cells), anti-CD 8 (T cytotoxic/suppressor), anti CD-20 (B plasma cells) and anti CD-68 (macrophages). Chronic periodontitis samples were mainly dominated by CD 4 and CD 8+ cells. On the contrary, in aggressive periodontitis patients all four cell types (CD 4, CD 8, CD 20 and CD 68 + cells, respectively) were remarkably increased. CD 20+ cells were significantly (p < 0.05) more prevalent in aggressive versus chronic periodontitis. The control samples expressed lower CD 4, CD 8, CD 20 and CD 68+ cells confirming a none inflammatory state. The present study demonstrates prevalence for CD 20+ cells in aggressive periodontitis lesions. However, further studies need to be performed to confirm and identify a clear pattern of inflammatory cells and hereafter the mechanisms sustaining the disease. |
| Databáze: | OpenAIRE |
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