Mechanical loading increases pro-inflammatory effects of nitrogen-containing bisphosphonate in human periodontal fibroblasts
| Autor: | Dan Meila, Heinrich Wehrbein, Collin Jacobs, Christian Walter, Cornelius Jacobs, Isabelle Dirks, Sabrina Schramm, Andreas Pabst |
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| Rok vydání: | 2017 |
| Předmět: |
Periodontal Ligament
medicine.medical_treatment Gene Expression Dentistry Enzyme-Linked Immunosorbent Assay Inflammation Pharmacology Real-Time Polymerase Chain Reaction Zoledronic Acid Dinoprostone 03 medical and health sciences 0302 clinical medicine Tensile Strength Gene expression medicine Humans Periodontal fiber MTT assay Viability assay Receptor General Dentistry Cells Cultured Tissue Inhibitor of Metalloproteinase-1 Diphosphonates Chemistry business.industry Activator (genetics) Interleukin-8 Imidazoles 030206 dentistry Fibroblasts Bisphosphonate Matrix Metalloproteinase 8 Cyclooxygenase 2 030220 oncology & carcinogenesis Stress Mechanical Clodronic Acid medicine.symptom business Biomarkers |
| Zdroj: | Clinical Oral Investigations. 22:901-907 |
| ISSN: | 1436-3771 1432-6981 |
| Popis: | There is increasing evidence that inflammation and biomechanical loading can influence the effects of bisphosphonates (BP). The aim of this study was to investigate the influence of tensile strain application combined with IL-1s and clodronate or zoledronate on human periodontal ligament fibroblasts (HPdLF) in vitro. HPdLF were cultured with 10 nM IL-1s and 5 μM clodronate or zoledronate for 48 h. Cells were applied to cyclic tensile strain (CTS; 3% elongation) for 12 h in vitro. Cell number was analyzed directly after CTS by MTT assay. Gene expression of receptor activator of cyclooxygenase-2 (COX-2) was investigated using real-time PCR. MMP-8, TIMP-1, and PGE2 were measured by ELISA. Statistics were performed with SPSS (ANOVA, p |
| Databáze: | OpenAIRE |
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