Ex situ bioengineering of bioartificial endocrine glands: A new frontier in regenerative medicine of soft tissue organs

Autor: D. Dallatana, Valentina Strusi, Anna Tampieri, Elena Bassoli, Ivan Martin, Andrea Gatto, Nicoletta Zini, Giulia Spaletta, Monica Sandri, Roberto Toni, Andrea Ferrari
Přispěvatelé: Toni R, Tampieri A, Zini N, Strusi V, Sandri M, Dallatana D, Spaletta G, Bassoli E, Gatto A, Ferrari A, Martin I.
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Annals of anatomy 193 (2011): 381–394.
info:cnr-pdr/source/autori:Toni R, Tampieri A, Zini N, Strusi V, Sandri M, Dallatana D, Spaletta G, Bassoli E, Gatto A, Ferrari A, Martin I./titolo:Ex situ bioengineering of bioartificial endocrine glands: a new frontier in regenerative medicine of soft tissue organs./doi:/rivista:Annals of anatomy/anno:2011/pagina_da:381/pagina_a:394/intervallo_pagine:381–394/volume:193
Annals of anatomy 193 (2011): 381–394. doi:10.1016/j.aanat.2011.06.004
info:cnr-pdr/source/autori:Toni, Roberto; Tampieri, Anna; Zini, Nicoletta; Strusi, Valentina; Sandri, Monica; Dallatana, Davide; Spaletta, Giulia; Bassoli, Elena; Gatto, Andrea; Ferrari, Andrea; Martin, Ivan/titolo:Ex situ bioengineering of bioartificial endocrine glands: A new frontier in regenerative medicine of soft tissue organs/doi:10.1016%2Fj.aanat.2011.06.004/rivista:Annals of anatomy/anno:2011/pagina_da:381/pagina_a:394/intervallo_pagine:381–394/volume:193
Popis: Ex situ bioengineering is one of the most promising perspectives in the field of regenerative medicine allowing for organ reconstruction outside the living body; i.e. on the laboratory bench. A number of hollow viscera of the cardiovascular, respiratory, genitourinary, and digestive systems have been successfully bioengineered ex situ, exploiting biocompatible scaffolds with a 3D morphology that recapitulates that of the native organ (organomorphic scaffold). In contrast, bioengineering of entire soft tissue organs and, in particular endocrine glands still remains a substantial challenge. Primary reasons are that no organomorphic scaffolding for endocrine viscera have as yet been entirely assembled using biocompatible materials, nor is there a bioreactor performance capable of supporting growth within the thickness range of the regenerating cell mass which has proven to be reliable enough to ensure formation of a complete macroscopic gland ex situ. Current technical options for reconstruction of endocrine viscera include either biocompatible 3D reticular scaffolds lacking any organomorphic geometry, or allogenic/xenogenic acellular 3D matrices derived from a gland similar to that to be bioengineered, eventually recellularized by autologous/heterologous cells. In 2007, our group designed, using biocompatible material, an organomorphic scaffold-bioreactor unit for bioengineering ex situ the human thyroid gland, chosen as a model for its simple anatomical organization (repetitive follicular cavities). This unit reproduces both the 3D native geometry of the human thyroid stromal/vascular scaffold, and the natural thyrocyte/vascular interface. It is now under intense investigation as an experimental tool to test cellular 3D auto-assembly of thyroid tissue and its related vascular system up to the ex situ generation of a 3D macroscopic thyroid gland. We believe that these studies will lay the groundwork for a new concept in regenerative medicine of soft tissue and endocrine organs: i.e. that the organomorphism of a biocompatible scaffold-bioreactor complex is essential to both the 3D organization of seeded stem cells/precursor cells and their phenotypic fate as glandular/parenchymal/vascular elements, eventually leading to a physiologically competent and immuno-tolerant bioconstruct, macroscopically suitable for transplantation and clinical applications. (C) 2011 Elsevier GmbH. All rights reserved.
Databáze: OpenAIRE
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