Increased Production of Interleukin-4, Interleukin-10, and Granulocyte-Macrophage Colony-Stimulating Factor by Type 2 Diabetes' Mononuclear Cells Infected with Dengue Virus, but Not Increased Intracellular Viral Multiplication
| Autor: | Ing-Kit Lee, Chang-Mei Chen, Yen-Hsu Chen, Ching-Jung Hsieh, Kuender D. Yang, Zih-Syuan Yang, Rong-Fu Chen, Chun-Yu Lin, Chung-Hao Huang, Chiung-Fen Liu, Jien-Wei Liu, Lin Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Article Subject Population Intracellular Space lcsh:Medicine Dengue virus Biology medicine.disease_cause Virus Replication Peripheral blood mononuclear cell General Biochemistry Genetics and Molecular Biology Dengue fever Dengue medicine Humans education Interleukin 4 Chemokine CCL2 Demography education.field_of_study General Immunology and Microbiology Tumor Necrosis Factor-alpha lcsh:R virus diseases Granulocyte-Macrophage Colony-Stimulating Factor General Medicine Dengue Virus Middle Aged Viral Load medicine.disease Virology Interleukin-10 Interleukin 10 Granulocyte macrophage colony-stimulating factor Diabetes Mellitus Type 2 Case-Control Studies Immunology Clinical Study Leukocytes Mononuclear Female Interleukin-4 Viral load medicine.drug |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2013 (2013) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | It has been reported that diabetes mellitus (DM) was an epidemiologically identified risk factor for development of dengue hemorrhagic fever (DHF)/severe dengue in dengue virus (DENV) affected patients, and T helper 2 (Th2) cytokines such as interleukin-4 (IL-4) and IL-10 each plays an important role in the immunopathogenesis of DHF in studies involving general population. To better understand the relationship between these epidemiological and immunological findings, we performed anin vitrostudy evaluating the sequential immunological reactions and viral load in the DENV infected mononuclear cells of adults with type 2 DM (T2DM group,n=33) and normal adults (control group,n=29). We found in the T2DM group significantly higher IL-4 level on the first(P=0.049)and the third(P=0.022)postinfection days, while higher IL-10(P=0.042)and granulocyte-macrophage colony-stimulating factor (GM-CSF)(P=0.009)were detected on the third postinfection day. No significant difference in DENV viral load between the cultured mononuclear cells from both groups was found on the first and third post-infection days. These data immunologically suggest that patients with T2DM are at higher risk for development of DHF/severe dengue and strengthen the previously epidemiologically identified role of DM being a predictive risk factor for progressing into DHF/severe dengue in DENV-affected patients. |
| Databáze: | OpenAIRE |
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