Adipose-Derived Stem-Cell-Seeded Non-Cross-Linked Porcine Acellular Dermal Matrix Increases Cellular Infiltration, Vascular Infiltration, and Mechanical Strength of Ventral Hernia Repairs
| Autor: | Lina W. Dunne, Kristin C. Turza, Justin Hubenak, Charles E. Butler, Tejaswi Iyyanki, Qixu Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
CD31
Acellular Dermis Pathology medicine.medical_specialty Swine Biomedical Engineering Adipose tissue Bioengineering Cell morphology Biochemistry Biomaterials Masson's trichrome stain Tissue engineering Cell Movement Tensile Strength Adipocytes medicine Animals Herniorrhaphy Tissue Engineering Tissue Scaffolds Guided Tissue Regeneration Chemistry Stem Cells Original Articles Equipment Design Skin Transplantation medicine.disease Rats Equipment Failure Analysis Cellular infiltration Cross-Linking Reagents Treatment Outcome Wound healing Stem Cell Transplantation |
| Zdroj: | Tissue Engineering Part A. 21:475-485 |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Adipose-derived stem cells (ASCs) facilitate wound healing by improving cellular and vascular recruitment to the wound site. Therefore, we investigated whether ASCs would augment a clinically relevant bioprosthetic mesh-non-cross-linked porcine acellular dermal matrix (ncl-PADM)-used for ventral hernia repairs in a syngeneic animal model. ASCs were isolated from the subcutaneous adipose tissue of Brown Norway rats, expanded, and labeled with green fluorescent protein. ASCs were seeded (2.5×10(4) cells/cm(2)) onto ncl-PADM for 24 h before surgery. In vitro ASC adhesion to ncl-PADM was assessed at 0.5, 1, and 2 h after seeding, and cell morphology on ncl-PADM was visualized by scanning electron microscopy. Ventral hernia defects (2×4 cm) were created and repaired with ASC-seeded (n=31) and control (n=32) ncl-PADM. Explants were harvested at 1, 2, and 4 weeks after surgery. Explant remodeling outcomes were evaluated using gross evaluation (bowel adhesions, surface area, and grade), histological analysis (hematoxylin and eosin and Masson's trichrome staining), immunohistochemical analysis (von Willebrand factor VIII), fluorescent microscopy, and mechanical strength measurement at the tissue-bioprosthetic mesh interface. Stem cell markers CD29, CD90, CD44, and P4HB were highly expressed in cultured ASCs, whereas endothelial and hematopoietic cell markers, such as CD31, CD90, and CD45 had low expression. Approximately 85% of seeded ASCs adhered to ncl-PADM within 2 h after seeding, which was further confirmed by scanning electron microcopy examination. Gross evaluation of the hernia repairs revealed weak omental adhesion in all groups. Ultimate tensile strength was not significantly different in control and treatment groups. Conversely, elastic modulus was significantly greater at 4 weeks postsurgery in the ASC-seeded group (p |
| Databáze: | OpenAIRE |
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