Triggering of Apoptosis is not Sufficient to Induce Human Immunodeficiency Virus Gene Expression
| Autor: | Puneet C. Aggarwal, Jacqueline D. Oakley, Kristoffer Valerie, Chad M. Hershey, Isam B. Estwani, Mohiuddin M. Taher |
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| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Infrared Rays Ultraviolet Rays p38 mitogen-activated protein kinases Blotting Western Clinical Biochemistry Apoptosis p38 Mitogen-Activated Protein Kinases Biochemistry Amino Acid Chloromethyl Ketones Jurkat Cells Cell Line Tumor Radiation Ionizing Gene expression Genetics Ultraviolet light medicine Humans Staurosporine Luciferases Molecular Biology Caspase A549 cell Dose-Response Relationship Drug biology Cytochrome c NF-kappa B HIV Dose-Response Relationship Radiation Hydrogen Peroxide Cell Biology Caspase Inhibitors Molecular biology Enzyme Activation Kinetics Gene Expression Regulation biology.protein Mitogen-Activated Protein Kinases Oligopeptides DNA Damage medicine.drug |
| Zdroj: | IUBMB Life (International Union of Biochemistry and Molecular Biology: Life). 55:415-427 |
| ISSN: | 1521-6551 1521-6543 |
| Popis: | We examined whether there is any causative link between apoptosis and HIV gene expression elicited in response to ultraviolet light (UV) and ionizing radiation (IR). We found that both UV and IR activate HIV gene expression in human T lymphoblastoid 1G5 (HIVluc) cells, but with different kinetics and magnitudes. Treatment with either type of radiation resulted in increased apoptosis, which correlated closely with HIV gene expression. The involvement of caspases in the IR response was demonstrated by using zVAD-FMK and zDEVD-FMK caspase inhibitors; both apoptosis and HIV gene expression were inhibited to similar extent. Surprisingly, treatment of 1G5 cells with FAS antibody triggered apoptosis but did not increase HIV gene expression. A correlation between increased apoptosis and gene expression was also demonstrated in human carcinoma HIVcat/A549 cells with UV whereas IR triggered apoptosis but did not activate HIV gene expression. Most significantly, UV activation of HIV gene expression, and NF- κB and p38 MAP kinase, both important for efficient HIV gene expression, were not affected by treatment with the zVAD-FMK and zDEVD-FMK inhibitors. Treatment of HIVcat/A549 cells with staurosporine or scrape-loading of cells with cytochrome c resulted in apoptosis but no increase in HIV gene expression. Altogether, a direct correlation exists between apoptosis and HIV gene expression in T-cells in response to both UV and IR but this is not the case in carcinoma cells. Triggering of apoptosis per se in either cell type does not necessarily result in increased HIV gene expression. Most importantly, the apoptotic and HIV gene expression responses elicited by UV are different to some extent and can be separated. IUBMB Life, 55: 415-427, 2003 |
| Databáze: | OpenAIRE |
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