Prognostic Value of T Cell Immunoglobulin Mucin-3 in Prostate Cancer
| Autor: | Zhehu Jin, Long-Zhen Piao, Xiu-Zhe Dong, Lian-Hua Zhu, Yong-Rui Piao |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Oncology PCA3 Cancer Research medicine.medical_specialty Small interfering RNA Lung Neoplasms Epidemiology T cell Blotting Western Apoptosis Bone Neoplasms Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Prostate cancer Internal medicine Biomarkers Tumor Tumor Cells Cultured medicine Humans RNA Messenger Hepatitis A Virus Cellular Receptor 2 Aged Cell Proliferation Neoplasm Staging Aged 80 and over Gene knockdown biology Reverse Transcriptase Polymerase Chain Reaction business.industry Cell growth Public Health Environmental and Occupational Health Membrane Proteins Prostatic Neoplasms Middle Aged Prognosis medicine.disease Survival Rate medicine.anatomical_structure Lymphatic Metastasis biology.protein Immunohistochemistry Neoplasm Grading Neoplasm Recurrence Local Antibody business Follow-Up Studies |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 14:3897-3901 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2013.14.6.3897 |
| Popis: | Background: Optimal treatment for prostate cancer remains a challenge worldwide. Recently, T cell immunoglobulin mucin-3 (TIM-3) has been implicated in tumor biology but its contribution prostate cancer remains unclear. The aim of this study was to investigate the role of TIM-3 as a prognostic marker in patients with prostate cancer. Methods: TIM-3 protein expression was determined by immunohistochemistry and Western blotting in 137 prostate cancer tumor samples and paired adjacent benign tissue. We also performed cell proliferation assays using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) and cell invasion assays. The effects of small interfering RNA (siRNA)-mediated knockdown of TIM-3 (TIM-3 siRNA) in two human prostate cancer cell lines were also evaluated. Results: TIM-3 expression was higher in prostate cancer tissue than in the adjacent benign tissue (P |
| Databáze: | OpenAIRE |
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