Gaiella occulta gen. nov., sp. nov., a novel representative of a deep branching phylogenetic lineage within the class Actinobacteria and proposal of Gaiellaceae fam. nov. and Gaiellales ord. nov
| Autor: | Milton S. da Costa, Luciana Albuquerque, Peter Schumann, Fred A. Rainey, Luís França, M. Fernanda Nobre |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Lineage (evolution) Molecular Sequence Data Biology DNA Ribosomal Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Species Specificity Genus RNA Ribosomal 16S Botany Groundwater Gene Phylogeny Ecology Evolution Behavior and Systematics chemistry.chemical_classification Portugal Phylogenetic tree Fatty Acids Sequence Analysis DNA 16S ribosomal RNA Bacterial Typing Techniques Amino acid Actinobacteria chemistry Peptidoglycan Diaminopimelic acid |
| Zdroj: | Systematic and Applied Microbiology. 34:595-599 |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/j.syapm.2011.07.001 |
| Popis: | Two isolates, with an optimum growth temperature of about 35–37 °C and an optimum pH for growth between 6.5 and 7.5, were recovered from a deep mineral water aquifer in Portugal. Strains form rod-shaped cells and were non-motile. These strains were non-pigmented, strictly aerobic, catalase and oxidase positive. Strains F2-233 T and F2-223 assimilated carbohydrates, organic acids and amino acids. Major fatty acids were novel iso internally branched such as 17:0 iso 10-methyl, 17:0 iso and 15:0 iso 8-methyl. The peptidoglycan contained meso -diaminopimelic acid and menaquinone MK-7 was the major respiratory quinone. Analysis of the 16S rRNA gene shows the strains to cluster with species of the genera Thermoleophilum , Patulibacter , Conexibacter and Solirubrobacter to which they have pairwise sequence similarity in the range 87–88%. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics we describe a new species of a novel genus represented by strain F2-233 T (=CECT 7815 T = LMG 26412 T ) for which we propose the name Gaiella occulta gen. nov., sp. nov. We also propose that this organism represents a novel family named Gaiellaceae fam. nov. of a novel order named Gaiellales ord. nov. |
| Databáze: | OpenAIRE |
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