Neutral Re(I) complex platform for live intracellular imaging
| Autor: | Shane M. Hickey, Phillip J. Wright, Sally E. Plush, Christie A. Bader, Todd A. Gillam, Stefano Zacchini, Doug A. Brooks, Chiara Caporale, Alexandra Sorvina, Janna L. Morrison, Robert Darren Brooks, Massimiliano Massi, Melissa V. Werrett, Stefano Stagni |
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| Přispěvatelé: | Gillam, Todd A, Caporale, Chiara, Brooks, Robert D, Bader, Christie A, Sorvina, Alexandra, Werrett, Melissa V, Wright, Phillip J, Morrison, Janna L, Massi, Massimiliano, Brooks, Doug A, Zacchini, Stefano, Hickey, Shane M, Stagni, Stefano, Plush, Sally E, Gillam T.A., Caporale C., Brooks R.D., Bader C.A., Sorvina A., Werrett M.V., Wright P.J., Morrison J.L., Massi M., Brooks D.A., Zacchini S., Hickey S.M., Stagni S., Plush S.E. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Azides
Endosome Rhenium Tetrazoles Intracellular Space metal complexes 010402 general chemistry 01 natural sciences Cell Line Inorganic Chemistry Live cell imaging Coordination Complexes Organelle Myocytes Cardiac Physical and Theoretical Chemistry Coordination Complexe 010405 organic chemistry Chemistry imaging platforms 0104 chemical sciences Molecular Imaging imaging agents Rhenium Azide Biophysics Molecular imaging Intracellular |
| Popis: | Luminescent metal complexes are a valuable platform for the generation of cell imaging agents. However, many metal complexes are cationic, a factor that can dominate the intracellular accumulation to specific organelles. Neutral Re(I) complexes offer a more attractive platform for the development of bioconjugated imaging agents, where charge cannot influence their intracellular distribution. Herein, we report the synthesis of a neutral complex (ReAlkyne), which was used as a platform for the generation of four carbohydrate-conjugated imaging agents via Cu(I)-catalyzed azide-alkyne cycloaddition. A comprehensive evaluation of the physical and optical properties of each complex is provided, together with a determination of their utility as live cell imaging agents in H9c2 cardiomyoblasts. Unlike their cationic counterparts, many of which localize within mitochondria, these neutral complexes have localized within the endosomal/lysosomal network, a result consistent with examples of dinuclear carbohydrate-appended neutral Re(I) complexes that have been reported. This further demonstrates the utility of these neutral Re(I) complex imaging platforms as viable imaging platforms for the development of bioconjugated cell imaging agents. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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