Bothrops jararacussu snake venom-induces a local inflammatory response in a prostanoid- and neutrophil-dependent manner
| Autor: | Karoline S. Aragão, Carlos W. S. Wanderley, Fernando Q. Cunha, Camila Meireles de Souza Silva, Raimundo C. Palheta-Junior, Ronaldo A. Ribeiro, Camila Fernandes, D.F.C. Morelo, F. Cosker, Gerly Anne de Castro Brito, Deysi Viviana Tenazoa Wong, Alexandre Havt, Rafael Matos Ximenes, Roberto C. P. Lima-Júnior |
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| Předmět: |
Neutrophils
Interleukin-1beta Inflammation Enzyme-Linked Immunosorbent Assay Pharmacology Toxicology Calcium in biology chemistry.chemical_compound Mice Edema Crotalid Venoms medicine Animals Humans Bothrops biology Chemistry Tumor Necrosis Factor-alpha Prostanoid Chemotaxis biology.organism_classification Mast cell Chemotaxis Leukocyte medicine.anatomical_structure Snake venom Cyclooxygenase 2 Immunology Prostaglandins Female medicine.symptom NEUTRÓFILOS |
| Zdroj: | Europe PubMed Central Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Local tissue reactions provoked by Bothrops venoms are characterized by edema, hemorrhage, pain, and inflammation; however, the mechanisms of tissue damage vary depending upon the species of snake. Here, we investigated the mechanisms involved in the local inflammatory response induced by the Bothrops jararacussu venom (BjcuV). Female Swiss mice were injected with either saline, BjcuV (0.125–8 μg/paw) or loratadine (an H1 receptor antagonist), compound 48/80 (for mast cell depletion), capsaicin (for C-fiber desensitization), infliximab (an anti-TNF-α antibody), indomethacin (a non-specific COX inhibitor), celecoxib (a selective COX-2 inhibitor) or fucoidan (a P- and L-selectins modulator) given before BjcuV injection. Paw edema was measured by plethysmography. In addition, paw tissues were collected for the measurement of myeloperoxidase activity, TNF-α and IL-1 levels, and COX-2 immunoexpression. The direct chemotactic effect of BjcuV and the in vitro calcium dynamic in neutrophils were also investigated. BjcuV caused an edematogenic response with increased local production of TNF-α and IL-1β as well as COX-2 expression. Both edema and neutrophil migration were prevented by pretreatment with indomethacin, celecoxib or fucoidan. Furthermore, BjcuV induced a direct in vitro neutrophil chemotaxis by increasing intracellular calcium. Therefore, BjcuV induces an early onset edema dependent upon prostanoid production and neutrophil migration. |
| Databáze: | OpenAIRE |
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