Expression of caspase-1 in synovial membrane-like interface tissue around loosened hip prostheses
| Autor: | Yrjö T. Konttinen, Mikko Liljeström, Mika Hukkanen, J. Westerlund, Jami Mandelin, Seppo Santavirta, T. Paavilainen, Tian-Fang Li |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Osteolysis Arthroplasty Replacement Hip Blotting Western Immunology Gene Dosage Caspase 1 Osteoarthritis Hip Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Rheumatology Stroma Gene expression Image Processing Computer-Assisted medicine Humans Immunology and Allergy RNA Messenger Caspase Aged 030304 developmental biology Aged 80 and over 030222 orthopedics 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction Synovial Membrane Interleukin Middle Aged medicine.disease Molecular biology Prosthesis Failure medicine.anatomical_structure Fluorescent Antibody Technique Direct biology.protein Female Hip Prosthesis Synovial membrane Interleukin-1 |
| Zdroj: | Rheumatology International. 22:97-102 |
| ISSN: | 1437-160X 0172-8172 |
| DOI: | 10.1007/s00296-002-0197-8 |
| Popis: | Caspase-1 expression in synovial membrane-like interface tissue (SMLIT) around loosened hip prostheses and osteoarthritic synovial samples was studied. Caspase-1 mRNA was found in SMLIT and synovial tissue. There is no difference in the copy numbers of caspase-1 mRNA between these samples. Both precursor and active forms of caspase-1 proteins appeared in these samples, but the number of positive cells was higher in SMLIT than in synovial tissue. Double labeling revealed that most caspase-1-positive cells were macrophages and fibroblasts. In the lining-like layers and deep stroma of SMLIT, many cells were double positive for active caspase-1 and interleukin-1 beta (IL-1beta). In contrast, the number of active caspase-1/IL-18 double-positive cells was very low. We conclude that caspase-1 synthesis is increased in SMLIT. Caspase-1 can be involved in implant loosening by processing IL-1beta precursor into its mature form, which is a potent osteoclast-activating factor and a major proinflammatory mediator. |
| Databáze: | OpenAIRE |
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