Adaptation of Staphylococcus aureus in a Medium Mimicking a Diabetic Foot Environment

Autor: Claude-Alexandre Gustave, Emmanuel Lemichez, Frédéric Laurent, Catherine Dunyach-Remy, Jean-Philippe Lavigne, Anne Tristan, Christelle Ngba-Essebe, Albert Sotto, Cassandra Pouget
Přispěvatelé: Virulence bactérienne et maladies infectieuses (VBMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de Reference des Staphylocoques, Université de Lyon, Toxines bactériennes - Bacterial Toxins, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Salvy-Córdoba, Nathalie
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Staphylococcus aureus
Health
Toxicology and Mutagenesis
nematode
MESH: Staphylococcal Infections
lcsh:Medicine
Virulence
MESH: Biofilms
adaptation
MESH: Virulence
Toxicology
medicine.disease_cause
biofilm
Microbiology
03 medical and health sciences
In vivo
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
medicine
MESH: Diabetic Foot
MESH: Wound Infection
MESH: Staphylococcus aureus
MESH: Immune Evasion
Gene
Pathogen
Caenorhabditis elegans
030304 developmental biology
0303 health sciences
MESH: Gene Expression Regulation
Bacterial
MESH: Humans
biology
030306 microbiology
lcsh:R
MESH: Time Factors
MESH: Energy Metabolism
Biofilm
biology.organism_classification
medicine.disease
Diabetic foot
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
virulence
in vitro model
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
diabetic foot infection
Panton–Valentin leukocidin
EDIN
Zdroj: Toxins
Volume 13
Issue 3
Toxins, 2021, 13 (3), pp.230. ⟨10.3390/toxins13030230⟩
Toxins, Vol 13, Iss 230, p 230 (2021)
ISSN: 2072-6651
DOI: 10.3390/toxins13030230
Popis: Staphylococcus aureus is the most prevalent pathogen isolated from diabetic foot infections (DFIs). The purpose of this study was to evaluate its behavior in an in vitro model mimicking the conditions encountered in DFI. Four clinical S. aureus strains were cultivated for 16 weeks in a specific environment based on the wound-like medium biofilm model. The adaptation of isolates was evaluated as follows: by Caenorhabditis elegans model (to evaluate virulence)
by quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) (to evaluate expression of the main virulence genes)
and by Biofilm Ring test® (to assess the biofilm formation). After 16 weeks, the four S. aureus had adapted their metabolism, with the development of small colony variants and the loss of b-hemolysin expression. The in vivo nematode model suggested a decrease of virulence, confirmed by qRT-PCRs, showing a significant decrease of expression of the main staphylococcal virulence genes tested, notably the toxin-encoding genes. An increased expression of genes involved in adhesion and biofilm was noted. Our data based on an in vitro model confirm the impact of environment on the adaptation switch of S. aureus to prolonged stress environmental conditions. These results contribute to explore and characterize the virulence of S. aureus in chronic wounds.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje