Effect of Polyamine on Pigmentation, Reactive Oxidative Species and Antioxidant under Drought in Maize (Zea mays L.)
| Autor: | Md. Golam Rasul, Mohammad Zakaria, Sharmin Akter, Md. Mahathir Sarker, Sudipta Dutta, Irin Sultana Nila, Md. Motiar Rohman, Md. Masudul Haque |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Glutathione reductase Polyethylene Glycol Antioxidants Superoxide dismutase lcsh:Agriculture 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Zea mays L AsA-GSH cycle medicine Homeostasis Food science lcsh:Agriculture (General) chemistry.chemical_classification biology Drought Glutathione peroxidase fungi lcsh:S food and beverages General Medicine Glutathione Ascorbic acid Abiotic stress lcsh:S1-972 Maize Spermidine 030104 developmental biology chemistry Oxidative stress biology.protein 030217 neurology & neurosurgery Peroxidase |
| Zdroj: | Turkish Journal of Agriculture: Food Science and Technology, Vol 6, Iss 7, Pp 799-811 (2018) |
| Popis: | To examine polyamines (PAs) effect in modulating the drought induced by polyethylene glycol (PEG) in maize (Zea mays L.) seedlings (variety Khoibhutta, 8 day seedlings grown in petri dish in incubator) were subjected to 20% PEG (polyethylene glycol) followed by 20 µmol PAs, Putrescine (Put), Spermidine (Spd) and Spermine (Spm) with PEG solution for 48 hours. Sharp decrease in Relative Water Content (RWC), Chl a, Chl b, carotenoid (Car) and total pigment content was observed under drought compared to control condition, while PAs application reversed their decreasing trends. PEG significantly increased Reactive Oxidative Species (ROS) [superoxide (O2•âˆ’) and H2O2], Methyl Glyoxal (MG), Melondialdehyde (MDA) and Lipoxigenase (LOX) activity, while Pas decreased the contents considerably (except MG) as compared to those under drought. Drought increased proline content, which was further augmented in PA treatments. PAs failed to incline glyoxalase‘s (Gly-I and Gly-II) activities, reduced under PEG. The activity and western blot confirmed the accumulation of Glutathione S-Transferase (GST) under drought, but PAs failed to augment the activity. Ascorbic Acid (AsA) and Glutathione (GSH) got oxidized into Dehydroascorbate (DHA) and oxidized Glutathione (GSSG) under drought but PAs effectively maintained homeostasis. Superoxide Dismutase (SOD), Peroxidase (POD), Ascorbate Peroxidase (APX), Glutathione Peroxidase (GPX), Monodehydroascorbatereductase (MDHAR), Dehydroascorbatereductase (DHAR), and Glutathione Reductase (GR) inclined in drought stressed seedlings, while Catalase (CAT) activity decreased under drought. PAs addition increased SOD, POD, GPX, CAT, MDHAR, and GR activities, but declined DHAR activity. These findings suggested important role of PAs in increasing tolerance under short term drought by modulating antioxidant effect. |
| Databáze: | OpenAIRE |
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