Widespread Tissue Expression of Gastrin-Binding-Protein Mrna
| Autor: | Rolf Håkanson, A. G. Nylander, Hans-Jürg Monstein |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Biology Polymerase Chain Reaction digestive system Biochemistry Mice chemistry.chemical_compound Multienzyme Complexes Sequence Homology Nucleic Acid Complementary DNA Animals Humans RNA Messenger Northern blot Autocrine signalling Receptor Gastrin chemistry.chemical_classification Messenger RNA Base Sequence Mitochondrial Trifunctional Protein digestive oral and skin physiology Blotting Northern Molecular biology Rats Enzyme chemistry Carrier Proteins hormones hormone substitutes and hormone antagonists DNA |
| Zdroj: | European Journal of Biochemistry. 246:502-507 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1997.00502.x |
| Popis: | Glycine-extended forms of gastrin (gastrin-Gly) are thought to be involved in the autocrine growth control of colorectal carcinomas. The recently described gastrin-binding protein has been suggested to be a gastrin-Gly accepting receptor. Northern blot analysis demonstrated the expression of gastrin-binding-protein mRNA in many tissues of mouse, rat, and man. The gastrin-binding-protein mRNA expression was confirmed by reverse-transcribed PCR analysis. Analysis of the cDNA and the deduced amino acid sequence of the PCR-amplified rat gastrin-binding-protein DNA fragments revealed sequence identity (except in a single position) with the corresponding human and pig gastrin-binding protein and with the alpha-subunit of a rat and human mitochondrial trifunctional enzyme, involved in fatty acid oxidation. The widespread and abundant tissue expression of gastrin-binding-protein mRNA and its sequence identity with a fatty-acid-oxidizing enzyme do not support the view that it represents a genuine gastrin receptor. |
| Databáze: | OpenAIRE |
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