The loss of endoglin promotes the invasion of extravillous trophoblasts
| Autor: | Hiroko Matsumura, Akira Iwase, Hiroyuki Tsuda, Takeshi Senga, Yukio Mano, Eiko Yamamoto, Fumitaka Kikkawa, Seiji Sumigama, Tomomi Kotani, Kiyozumi Shibata |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Placenta Cell Receptors Cell Surface Cell Line Small hairpin RNA Transforming Growth Factor beta1 Endocrinology Transforming Growth Factor beta3 Antigens CD Cell Movement Pregnancy hemic and lymphatic diseases Internal medicine otorhinolaryngologic diseases medicine Humans Cell Proliferation Gene knockdown Chemistry Cell growth Endoglin Trophoblast Transfection Urokinase-Type Plasminogen Activator Matrix Metalloproteinases Trophoblasts medicine.anatomical_structure Cell culture Immunology Cancer research Female |
| Zdroj: | Endocrinology. 152(11) |
| ISSN: | 1945-7170 |
| Popis: | Endoglin is a coreceptor for TGF-β, which is expressed in syncytiotrophoblasts. The soluble form of endoglin (sEng) has been observed to increase in the serum of preeclamptic patients. Several studies have shown that endoglin is involved in cancer invasion. However, the role of endoglin in extravillous trophoblasts (EVT), which have an invasive phenotype, remains unknown. The present study was designed to investigate the expression and role of endoglin in human EVT. We found that endoglin was mainly expressed on cytotrophoblasts within the cell column during the first trimester and its expression decreased in the EVT by immunohistochemistry and immunocytochemistry. The expression of endoglin significantly increased after treatment with TGF-β1 and TGF-β3 in the human EVT cell line, HTR-8/SVneo, as detected by semiquantitative RT-PCR. To investigate the role of endoglin in EVT, the stable knockdown of endoglin was performed by lentiviral short hairpin RNA transfection into the HTR-8/SVneo cells. Although proliferation was not affected, the motility and invasiveness of the HTR-8/SVneo cells significantly increased by the knockdown of endoglin. Both the mRNA expression and secretion of urokinase-type plasminogen activator significantly increased in endoglin knockdown cells. The secretion of sEng was very low in HTR-8/SVneo, and the treatment of endoglin knockdown cells with 10 ng/ml sEng had no effect on their invasiveness. Therefore, the suppression of sEng was not involved in the increased invasiveness of endoglin knockdown cells. These results suggested that EVT increased their invasive function as a result of decreasing expression of transmembrane endoglin. |
| Databáze: | OpenAIRE |
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