Staphylococcus aureus SaeR/S-regulated factors reduce human neutrophil reactive oxygen species production
| Autor: | Joseph Azzolino, Jos A. G. van Strijp, Kyler B. Pallister, Jovanka M. Voyich, Nienke W.M. de Jong, Conrad B. Addison, Fermin E. Guerra |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Hypochlorous acid Neutrophils Immunology Biology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Phagocytosis Superoxides medicine Journal Article Humans Immunology and Allergy Pathogen Respiratory Burst chemistry.chemical_classification Innate immunity Reactive oxygen species Innate immune system Neutrophil clearance Virulence Bacteria Host-pathogen interactions Superoxide Gene Expression Regulation Bacterial Hydrogen Peroxide Cell Biology Catalase Hypochlorous Acid 030104 developmental biology chemistry Two-component systems biology.protein Luminol Primary Research Reactive Oxygen Species Protein Kinases Transcription Factors |
| Zdroj: | Journal of Leukocyte Biology, 100(5), 1005. FASEB |
| ISSN: | 0741-5400 |
| DOI: | 10.1189/jlb.4vmab0316-100rr |
| Popis: | Neutrophils are the first line of defense after a pathogen has breached the epithelial barriers, and unimpaired neutrophil functions are essential to clear infections. Staphylococcus aureus is a prevalent human pathogen that is able to withstand neutrophil killing, yet the mechanisms used by S. aureus to inhibit neutrophil clearance remain incompletely defined. The production of reactive oxygen species (ROS) is a vital neutrophil antimicrobial mechanism. Herein, we test the hypothesis that S. aureus uses the SaeR/S two-component gene regulatory system to produce virulence factors that reduce neutrophil ROS production. With the use of ROS probes, the temporal and overall production of neutrophil ROS was assessed during exposure to the clinically relevant S. aureus USA300 (strain LAC) and its isogenic mutant LACΔsaeR/S. Our results demonstrated that SaeR/S-regulated factors do not inhibit neutrophil superoxide (O2−) production. However, subsequent neutrophil ROS production was significantly reduced during exposure to LAC compared with LACΔsaeR/S. In addition, neutrophil H2O2 production was reduced significantly by SaeR/S-regulated factors by a mechanism independent of catalase. Consequently, the reduction in neutrophil H2O2 resulted in decreased production of the highly antimicrobial agent hypochlorous acid/hypochlorite anion (HOCl/−OCl). These findings suggest a new evasion strategy used by S. aureus to diminish a vital neutrophil antimicrobial mechanism. |
| Databáze: | OpenAIRE |
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