Culture and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Proven Mycobacterium Tuberculosis Endophthalmitis: A Case Series
| Autor: | K Lily Therese, Mamta Agarwal, Gayathri Ramasubban, Kushal Delhiwala, Ekta Rishi, Pradeep Susvar, Pukhraj Rishi, Tarun Sharma, Vishal R. Sharma, A.E. George, Jyotirmay Biswas, Pramod Bhende |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 030231 tropical medicine Eye Infections Bacterial law.invention Microbiology Mycobacterium tuberculosis Young Adult 03 medical and health sciences 0302 clinical medicine Endophthalmitis Bacterial Proteins law medicine Humans Immunology and Allergy RNA Messenger Tuberculosis Pulmonary Polymerase chain reaction Retrospective Studies Antigens Bacterial Bacteriological Techniques biology Reverse Transcriptase Polymerase Chain Reaction business.industry RNA Middle Aged bacterial infections and mycoses medicine.disease biology.organism_classification Virology Reverse transcriptase Staining Vitreous Body Ophthalmology Real-time polymerase chain reaction 030221 ophthalmology & optometry Female business Nested polymerase chain reaction Acyltransferases |
| Zdroj: | Ocular Immunology and Inflammation. 26:220-227 |
| ISSN: | 1744-5078 0927-3948 |
| DOI: | 10.1080/09273948.2016.1207786 |
| Popis: | Purpose: To report early confirmation of Mycobacterium tuberculosis (MTB) endophthalmitis by detection of 85B mRNA in vitreous by a reverse transcriptase polymerase chain reaction (RT-PCR) technique.Methods: Retrospective, interventional case series of 5 patients with MTB endogenous endophthalmitis. Vitreous aspirate was subjected to Ziehl-Neelsen (ZN) staining, BACTEC MicroMGIT culture, RT-PCR targeting the 85B gene, real-time PCR targeting the IS6110 region, and nested PCR targeting the MPB64 gene and IS6110 region. Correlation between detection of MTB RNA, culture positivity, and ZN staining was studied.Results: Five patients with endophthalmitis with no history of tuberculosis revealed acid-fast bacilli on ZN staining of vitreous. RT-PCR detected 85B RNA within 24 h. Culture for MTB was positive in 3/5 patients after 1 month. None of the eyes recovered any useful vision.Conclusions: RT-PCR can detect viable MTB RNA and provide evidence of active infection much earlier than culture. |
| Databáze: | OpenAIRE |
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