Emerging high-risk ST101 and ST307 carbapenem-resistant Klebsiella pneumoniae clones from bloodstream infections in Southern Italy
Autor: | Antonio Parisi, Angelica Bianco, Anna Morea, Maria Chironna, Daniela Loconsole, Loredana Capozzi, Anna Lisa De Robertis, Rosanna Mallamaci, Marisa Accogli, Michele Quarto |
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Rok vydání: | 2020 |
Předmět: |
Male
Klebsiella pneumoniae lcsh:QR1-502 Virulome Drug resistance Resistome lcsh:Microbiology Medical microbiology Drug Resistance Multiple Bacterial Prospective Studies Child Aged 80 and over Molecular Epidemiology 0303 health sciences General Medicine Middle Aged ST101 Electrophoresis Gel Pulsed-Field Infectious Diseases Italy Child Preschool ST307 Female Adult Microbiology (medical) medicine.medical_specialty Adolescent Biology Real-Time Polymerase Chain Reaction Serogroup beta-Lactamases lcsh:Infectious and parasitic diseases Microbiology Young Adult 03 medical and health sciences Bacterial Proteins medicine Humans lcsh:RC109-216 Typing Genotyping Aged 030304 developmental biology Whole genome sequencing 030306 microbiology Research lcsh:RM1-950 Infant Newborn Infant biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Klebsiella Infections lcsh:Therapeutics. Pharmacology Parasitology Carbapenem-resistant Klebsiella pneumoniae Multilocus sequence typing Bloodstream infections Multilocus Sequence Typing |
Zdroj: | Annals of Clinical Microbiology and Antimicrobials, Vol 19, Iss 1, Pp 1-10 (2020) Annals of Clinical Microbiology and Antimicrobials |
ISSN: | 1476-0711 |
Popis: | Background Carbapenem-resistant Klebsiella pneumoniae (CR-KP) is an urgent public health issue in Italy. This pattern of resistance is due mainly to dissemination of carbapenemase genes. Molecular characterization of carbapenem-resistant Klebsiella pneumoniae (CR-KP) strains was performed over a three-year period. In-depth analysis was performed on a subset of emerging CR-KP ST101 and ST307 clones. Methods A prospective study was performed on 691 patients with CR-KP bloodstream infections hospitalized in 19 hospitals located in three large provinces in Southern Italy. Carbapenemase genes were identified via genotyping methods. Multi-locus sequence typing (MLST) and Whole Genome Sequencing (WGS) were carried out on ST101 and ST307 isolates. Results Among the CR-KP isolates, blaKPC was found in 95.6%, blaVIM was found in 3.5%, blaNDM was found in 0.1% and blaOXA-48 was found in 0.1%. The blaKPC-3 variant was identified in all 104 characterized KPC-KP isolates. MLST of 231 representative isolates revealed ST512 in 45.5%, ST101 in 20.3% and ST307 in 18.2% of the isolates. cgMLST of ST307 and ST101 isolates revealed presence of more than one beta-lactam resistance gene. Amino acid substitution in the chromosomal colistin-resistance gene pmrB was found in two ST101 isolates. Conclusions ST512 is widespread in Southern Italy, but ST101 and ST307 are emerging since they were found in a significant proportion of cases. Aggressive infection control measures and a continuous monitoring of these high-risk clones are necessary to avoid rapid spread of CR-KP, especially in hospital settings. |
Databáze: | OpenAIRE |
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