Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes
| Autor: | G C Donaldson, N L Glass |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Polymerase Chain Reaction Applied Microbiology and Biotechnology Genome Aspergillus nidulans law.invention Ascomycota law Gene Conserved Sequence Phylogeny Polymerase chain reaction DNA Primers Genetics Base Sequence Neurospora crassa Ecology biology Basidiomycota Gene Amplification biology.organism_classification Restriction enzyme genomic DNA Genes Bacterial Mitosporic Fungi Primer (molecular biology) Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 61:1323-1330 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.61.4.1323-1330.1995 |
| Popis: | We constructed nine sets of oligonucleotide primers on the basis of the results of DNA hybridization of cloned genes from Neurospora crassa and Aspergillus nidulans to the genomes of select filamentous ascomycetes and deuteromycetes (with filamentous ascomycete affiliations). Nine sets of primers were designed to amplify segments of DNA that span one or more introns in conserved genes. PCR DNA amplification with the nine primer sets with genomic DNA from ascomycetes, deuteromycetes, basidiomycetes, and plants revealed that five of the primer sets amplified a product only from DNA of the filamentous ascomycetes and deuteromycetes. The five primer sets were constructed from the N. crassa genes for histone 3, histone 4, beta-tubulin, and the plasma membrane ATPase. With these five primer sets, polymorphisms were observed in both the size of and restriction enzyme sites in the amplified products from the filamentous ascomycetes. The primer sets described here may provide useful tools for phylogenetic studies and genome analyses in filamentous ascomycetes and deuteromycetes (with ascomycete affiliations), as well as for the rapid differentiation of fungal species by PCR. |
| Databáze: | OpenAIRE |
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