| Autor: |
Danielli Azevedo Monico, Italo Rodrigo Calori, Carla Souza, Enilza Maria Espreafico, Hong Bi, Antonio Claudio Tedesco |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Experimental cell research. 417(1) |
| ISSN: |
1090-2422 |
| Popis: |
Melanoma spheroid-loaded 3D skin models allow for the study of crucial tumor characteristics and factors at a superior level because the neoplastic cells are integrated into essential human skin components, permitting tumor-skin model communication. Herein, we designed a melanoma-containing artificial dermis by inserting multicellular tumor spheroids from the metastatic phase of WM 1617 melanoma cells into an artificial dermis. We cultured multicellular melanoma spheroids by hanging drop method (250 cells per drop) with a size of 420 μm in diameter after incubation for 14 days. These spheroids were integrated into the dermal equivalents that had been previously preparedwith a type-I collagen matrix and healthy fibroblasts. The melanoma spheroid cells invaded and proliferated in the artificial dermis. Spheroids treated with a 1.0 μmol/L aluminum chloride phthalocyanine nanoemulsion in the absence of light showed high cell viability. In contrast, under irradiation with visible red light (660 nm) at 25 J/cm |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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