Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology
| Autor: | Duangporn Kantachote, Julalak Chuprom, Mehraj Ahmad, Preeyanuch Bovornreungroj, Sawitree Dueramae |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Central composite design medicine.medical_treatment lcsh:Biotechnology Industrial fermentation Halobacterium 01 natural sciences Applied Microbiology and Biotechnology Article 03 medical and health sciences Halophilic protease Response surface methodology 010608 biotechnology lcsh:TP248.13-248.65 medicine Protease Chromatography biology Strain (chemistry) biology.organism_classification Halophile 030104 developmental biology Biochemistry Gelatin Fermentation Halobacterium sp. strain Biotechnology |
| Zdroj: | Biotechnology Reports, Vol 10, Iss C, Pp 17-28 (2016) Biotechnology Reports |
| ISSN: | 2215-017X |
| DOI: | 10.1016/j.btre.2016.02.004 |
| Popis: | Highlights • Halophilic protease producing Halobacterium sp. strain LBU50301 was isolated. • RSM optimized the fermentation conditions to enhance halophilic protease yield. • Optimized conditions used in bioreactor resulted about 13-fold enhancement. A new potent halophilic protease producer, Halobacterium sp. strain LBU50301 was isolated from salt-fermented fish samples (budu) and identified by phenotypic analysis, and 16S rDNA gene sequencing. Thereafter, sequential statistical strategy was used to optimize halophilic protease production from Halobacterium sp. strain LBU50301 by shake-flask fermentation. The classical one-factor-at-a-time (OFAT) approach determined gelatin was the best nitrogen source. Based on Plackett–Burman (PB) experimental design; gelatin, MgSO4·7H2O, NaCl and pH significantly influenced the halophilic protease production. Central composite design (CCD) determined the optimum level of medium components. Subsequently, an 8.78-fold increase in corresponding halophilic protease yield (156.22 U/mL) was obtained, compared with that produced in the original medium (17.80 U/mL). Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values. An overall 13-fold increase in halophilic protease yield was achieved using a 3 L laboratory fermenter and optimized medium (231.33 U/mL). |
| Databáze: | OpenAIRE |
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