ATP Synthesis and Biosensing Coupled to the Electroenzymatic Activity of a Hydrogenase on an Electrode/Biomimetic Membrane Interface
| Autor: | Marta C. Marques, Iván López-Montero, Marcos Pita, Antonio L. De Lacey, Ileana F. Márquez, Sonia Zacarias, Paolo Natale, Marisela Vélez, Cristina Gutierrez-Sanchez, Gabriel García-Molina, Inês A. C. Pereira |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España), Fundação para a Ciência e a Tecnologia (Portugal), European Research Council |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0209 industrial biotechnology
Hydrogenase lcsh:A 02 engineering and technology 01 natural sciences 020901 industrial engineering & automation Química física Lipid bilayer Electrochemical gradient chemistry.chemical_classification ATP synthase biology 010401 analytical chemistry 6. Clean water 0104 chemical sciences 3. Good health Coupling (electronics) n/a Enzyme Membrane chemistry biology.protein Biophysics lcsh:General Works Biosensor |
| Zdroj: | E-Prints Complutense. Archivo Institucional de la UCM instname Digital.CSIC. Repositorio Institucional del CSIC Proceedings, Vol 1, Iss 8, p 766 (2017) E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid |
| Popis: | This article belongs to the Proceedings of the 5th International Symposium on Sensor Science (I3S 2017). Cells generate energy by coupling a proton gradient across a phospholipid bilayer membrane with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an artificial environment, we show that ATP can be efficiently produced starting from molecular hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]- hydrogenase from Desulfovibrio vulgaris Hildenborough [1]. The electro-enzymatic oxidation of H2 generated a proton gradient across the supported biomimetic membrane that can be coupled to the in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system [2]. Such a system is also suitable for developing an electrochemical biosensor of ATP. This research was funded by the Spanish MINECO (Project CTQ2012-32448 and CTQ2015-71290-R) and by the Fundacao para a Ciencia e a Tecnologia (project PTDC/BBB- EP/0934/2012 and UID/Multi/04551/2013). I.L.-M acknowledges the European Research Council (ERC), grant no. ERC-StG-2013-338133 titled “MITOCHON” and RyC-2013-12609 from the Spanish MINECO. |
| Databáze: | OpenAIRE |
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