Floral nectar chitinase is a potential marker for monofloral honey botanical origin authentication:a case study from loquat (Eriobotrya japonica Lindl.)
Autor: | Richard I. Milne, Yue-Qin Song, Hong-Guang Zha, Jiang-Yu Fang, Xue-Long Ma, Hong-Xia Zhou |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Proteomics
Plant Nectar Brassica Flowers Chitinase activity Eriobotrya 01 natural sciences Mass Spectrometry Japonica Botanical origin Analytical Chemistry 0404 agricultural biotechnology Eriobotrya japonica Lindl Floral nectar Botany Nectar protein Animals Nectar Electrophoresis Gel Two-Dimensional Camellia sinensis Authentication biology Chitinases 010401 analytical chemistry Chitinase Honey 04 agricultural and veterinary sciences General Medicine Bees biology.organism_classification 040401 food science 0104 chemical sciences Thaumatin biology.protein Biomarkers Monofloral honey Food Science |
Zdroj: | Song, Y, I Milne, R, Zhou, H, Ma, X, Fang, J & Zha, H 2019, ' Floral nectar chitinase is a potential marker for monofloral honey botanical origin authentication : a case study from loquat ( Eriobotrya japonica Lindl.) ', Food Chemistry, vol. 282, pp. 76-83 . https://doi.org/10.1016/j.foodchem.2018.12.107 |
Popis: | Honey,as a commercial product, is a target of adulteration through inappropriate production practices and deliberate mislabelling of botanical origin. Floral nectar protein could be a good marker for determining the source flowers of honey, especially monofloral honeys. Here, nectar and monofloral honey from Eriobotrya japonica Lindl. (loquat) were systematically compared, especially regarding proteomic and enzymatic activity. Using two-dimensional electrophoresis and mass spectrometry, only bee-originated proteins were detected in loquat honey. Xylosidase, thaumatin, and two kinds of chitinases were detected in loquat floral nectar, and their activity in loquat nectar and honey were quantified. Following gel electrophoresis, loquat honey had similar chitinase activity profiles to loquat nectar, but both were clearly distinguishable from Camellia sinensis nectar and Brassica napus honey. To our knowledge, this is the first examination of nectar-origin enzyme activity in honey. Zymography of chitinases is a potential marker for determining or authenticating the botanical origin of honeys. |
Databáze: | OpenAIRE |
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