Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs
| Autor: | Syahrilnizam Abdullah, Suleiman Yusuf Alhaji, Fatma Hassan, Ezharul Houque Chowdhury, Rozita Rosli |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Article Subject
Transgene Nuclease Protection Assays Gene Expression lcsh:Medicine Electrophoretic Mobility Shift Assay Gene delivery Biology Transfection General Biochemistry Genetics and Molecular Biology Genes Reporter Apatites Cell Line Tumor Gene expression Animals Humans Particle Size Luciferases Cytotoxicity Lung Electrophoresis Agar Gel Mice Inbred BALB C Reporter gene Cell Death General Immunology and Microbiology lcsh:R Gene Transfer Techniques DNA General Medicine Molecular biology In vitro Cell culture Nanoparticles Research Article Plasmids |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells. Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs. This study aimed to (1) assess the gene delivery efficiency of CO3Apin vitroand in mouse lungs, (2) evaluate the cytotoxicity effect of CO3Ap/pDNAin vitro, and (3) characterize the CO3Ap/pDNA complex formulations. A significantly high level of reporter gene expression was detected from the lung cell line transfected with CO3Ap/pDNA complex prepared in both serum and serum-free medium. Cytotoxicity analysis revealed that the percentage of the viable cells treated with CO3Ap to be almost similar to the untreated cells. Characterization analyses showed that the CO3Ap/pDNA complexes are in a nanometer range with aggregated spherical structures and tended to be more negatively charged. In the lung of mice, highest level of transgene expression was observed when CO3Ap (8 μL) was complexed with 40 μg of pDNA at day 1 after administration. Although massive reduction of gene expression was seen beyond day 1 post administration, the level of expression remained significant throughout the study period. |
| Databáze: | OpenAIRE |
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