Noninvasive detection of human dehydroepiandrosterone, progesterone and testosterone using LC-MS/MS revealed effects of birth control pills/devices and body weight on ovulatory prediction
| Autor: | David C. Spink, Zhimin Tim Cao, Lei Shen, Edelgard Wulfert, Stephanie E. Wemm, Liqiao Han |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Ovulation medicine.medical_specialty Saliva Birth control pills Adolescent medicine.medical_treatment Clinical Biochemistry Dehydroepiandrosterone Body weight 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Sensitivity and Specificity Analytical Chemistry Steroid 03 medical and health sciences Young Adult 0302 clinical medicine Tandem Mass Spectrometry Internal medicine Lc ms ms medicine Humans Testosterone Progesterone Chromatography Chemistry 010401 analytical chemistry Body Weight Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Endocrinology Predicting ovulation Linear Models Ovulation Prediction Female Chromatography Liquid Contraceptives Oral |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1174 |
| ISSN: | 1873-376X |
| Popis: | Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used to measure steroids in human saliva. We studied the performance of a conventional LC-MS/MS for measuring dehydroepiandrosterone (DHEA), testosterone and progesterone in human saliva. These three steroids were co-extracted by liquid-liquid extraction and derivatized. Derivatives were resolved on a C18 column and quantified using an LC-MS/MS (AB Sciex API 2000) instrument. The assay’s limits of quantification were 0.03 ng/mL for all three steroids. Inter-assay coefficients of variation were 16.6–18.8% (DHEA), 12.0–15.8% (testosterone), and 12.7–19.3% (progesterone). Assay linearity analysis showed R2 of 0.9926, 0.9750 and 0.9949 for DHEA, testosterone and progesterone, respectively. No carry-over between samplings was observed. An ion-enhancement effect of 11.6% for DHEA determination and ion-suppression effects of 13.9% and 20.7% for analysis of progesterone and testosterone, respectively, were determined. No interferences by 9 steroid analogs were detected. Spiked recoveries were 85.5% (DHEA), 86.5% (testosterone), and 92.6% (progesterone). Comparison with laboratory developed test (LDT)-LC-MS/MS methods by other New York State Department of Health certified laboratories revealed R2 = 0.9425 (DHEA, LC-MS/MS = 1.0267 LDT + 21.989), R2 = 0.9849 (testosterone, LC-MS/MS = 0.9447 LDT + 9.8037), and R2 = 0.9736 (progesterone, LC-MS/MS = 1.1196 LDT + 0.0985). Reference intervals for the 3 steroids in saliva for young males and females were estimated. Results of intra-individual salivary progesterone analysis indicated that caution should be exercised when using progesterone concentrations in predicting ovulation for females who are under treatment with birth control pills/devices or has body a weight of > 90 kg. |
| Databáze: | OpenAIRE |
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