Human IgM Anti-IgM Cytotoxin for B Lymphocytes
| Autor: | PaulI. Terasaki, Cicciarelli J, E. V. Barnett, David Chia, Shirahama S |
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| Rok vydání: | 2008 |
| Předmět: |
Cytotoxicity
Immunologic B-Lymphocytes biology Cytotoxins Immunology Acid elution General Medicine Anti igm Biochemistry Molecular biology Absorption Antibodies Anti-Idiotypic Sepharose Immunoglobulin M Immunoglobulin G Genetics biology.protein Humans Immunology and Allergy Cytotoxic T cell Immune reactivity Binding Sites Antibody Antibody Cytotoxicity Serum Albumin |
| Zdroj: | Tissue Antigens. 15:275-282 |
| ISSN: | 1399-0039 0001-2815 |
| Popis: | B lymphocytes were shown previously to be killed at 5 degrees C by some autologous and allogeneic human sera. We show here that such cold cytotoxins are directed against immunoglobulins on the surface of B lymphocytes. The activity is partially removed by passing serum through IgG-coupled Sepharose and usually completely removed by passing serum through IgM-coupled Sepharose. Activity is regained from the columns by acid elution and IgM inhibits the cytotoxicity of these eluates. 125I-labeled eluates bind to IgG- and IgM-coupled Sepharose beads and binding is inhibited by IgM and to a lesser degree by IgG thus showing a primarily more avid binding to IgM as compared to IgG. Furthermore, the 125I-labeled cytotoxic eluates recognize the same determinant(s) on IgM and IgG. We suggest tht IgM anti-IgM antiimmunoglobulin may regulate immune reactivity by binding to B-lymphocyte surfaces. |
| Databáze: | OpenAIRE |
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