Differentiation of human leukemias in response to 12-0- tetradecanoylphorbol-13-acetate in vitro

Autor:	Beverly J. Lange, Richard A. Cooper, Alessandro Levis, Janet L. Abrahm, Giovanni Giac. Rovera, Luigi Pegoraro, Pacifico Meo
Rok vydání:	1980
Předmět:	Myeloid Phagocytosis Lymphoblastic Leukemia Immunology Myeloid leukemia Cell Biology Hematology Adhesion Biology Biochemistry Esterase In vitro medicine.anatomical_structure medicine Cancer research After treatment
Zdroj:	Blood. 55:859-862
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v55.5.859.859
Popis:	Leukemic cells from patients with acute myeloid leukemia underwent morphological, functional, and histochemical changes within 24–48 hr after treatment with 1.6 x 10–18 M 12–0-tetradecanoylphorbol-13-acetate (TPA). The changes included adhesion to the plastic substrate, a 4–6- fold increase in the number of phagocytic cells, and an increase in the number of alpha-naphthyl-acetate esterase (alpha-NAE) positive cells. In contrast, TPA treatment of cells from patients with acute lymphoblastic leukemia caused some aggregation of cells in suspension, but no changes in adhesion, phagocytosis, or alpha-NAE. Of the four cases of undifferentiated or unclassified leukemias studied, two failed to respond to TPA, one responded with a myeloid (adhesion) pattern, and one with a lymphoid (aggregation) pattern. These data suggest that leukemic myeloblasts retain the ability to express a variety of differentiated functions, and in some cases, it may be possible to use TPA as a tool to test the differentiative potential of undifferentiated human leukemias.
Databáze:	OpenAIRE
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