Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
| Autor: | Thijn R. Brummelkamp, Brent H. Cochran, Irene Wuethrich, Carla P. Guimaraes, Jan E. Carette, Annie S. Park, Maciej Kotecki, Malini Varadarajan, Eric Spooner, Alzbeta Godarova, Hidde L. Ploegh |
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| Rok vydání: | 2009 |
| Předmět: |
Monosaccharide Transport Proteins
Cytolethal distending toxin Virulence Factors Bacterial Toxins Molecular Sequence Data ved/biology.organism_classification_rank.species Exotoxins Mutagenesis (molecular biology technique) Haploidy Biology Insertional mutagenesis chemistry.chemical_compound Influenza A Virus H1N1 Subtype Peptide Elongation Factor 2 Cell Line Tumor Humans Diphtheria Toxin Histidine Amino Acid Sequence Genetic Testing Model organism Gene ADP Ribose Transferases Genetics Diphtheria toxin Adenosine Diphosphate Ribose Antigens Bacterial N-Acylneuraminate Cytidylyltransferase Multidisciplinary ved/biology Diphthamide Proteins Methyltransferases Biosynthetic Pathways Mutagenesis Insertional Genes chemistry Host-Pathogen Interactions Carboxylic Ester Hydrolases Genetic screen |
| Zdroj: | Science. 326:1231-1235 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1178955 |
| Popis: | “Haploid Human” Genetic screens can provide direct insight into biological processes that are poorly understood. Carette et al. (p. 1231 ) describe genetic screens using large-scale gene disruption in human cells haploid for all chromosomes except for chromosome 8. One screen was used to identify host factors essential for the activity of cytolethal distending toxin, a toxin found in several pathogenic bacteria. Another screen identified host gene products essential for infection with influenza, and an additional screen revealed genes required for the action of adenosine 5′-diphosphate (ADP)–ribosylating bacterial toxins. This loss-of-function genetic approach in mammalian cells will be widely applicable to study a variety of biological processes and cellular functions. |
| Databáze: | OpenAIRE |
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