CHE-3, a Cytosolic Dynein Heavy Chain, Is Required for Sensory Cilia Structure and Function in Caenorhabditis elegans
Autor: | H. G. A. M. van Luenen, Ronald H.A. Plasterk, S. R. Wicks, C. J. de Vries |
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Přispěvatelé: | Other departments |
Rok vydání: | 2000 |
Předmět: |
Male
Gene isoform Recombinant Fusion Proteins cilium Green Fluorescent Proteins Molecular Sequence Data Dynein Disorders of Sex Development nonmotile IFT Flagellum Polymerase Chain Reaction Motor protein taste Intraflagellar transport Animals Humans Protein Isoforms Amino Acid Sequence Cilia Cloning Molecular chemotaxis Caenorhabditis elegans Molecular Biology Crosses Genetic Phylogeny Neurons Genetics dynein Sequence Homology Amino Acid biology Cilium Dyneins DHC1b Cell Biology biology.organism_classification Cell biology Luminescent Proteins Mutagenesis Insertional Female flagella Sequence Alignment Genetic screen Developmental Biology |
Zdroj: | Developmental biology, 221(2), 295-307. Academic Press Inc. |
ISSN: | 0012-1606 |
DOI: | 10.1006/dbio.2000.9686 |
Popis: | Forward genetic screens using novel assays of nematode chemotaxis to soluble compounds identified three independent transposon-insertion mutations in the gene encoding the Caenorhabditis elegans dynein heavy chain (DHC) 1b isoform. These disruptions were mapped and cloned using a newly developed PCR-based transposon display. The mutations were demonstrated to be allelic to the che-3 genetic locus. This isoform of dynein shows temporally and spatially restricted expression in ciliated sensory neurons, and mutants show progressive developmental defects of the chemosensory cilia. These results are consistent with a role for this motor protein in the process of intraflagellar transport; DHC 1b acts in concert with a number of other proteins to establish and maintain the structural integrity of the ciliated sensory endings in C. elegans. |
Databáze: | OpenAIRE |
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