CHE-3, a Cytosolic Dynein Heavy Chain, Is Required for Sensory Cilia Structure and Function in Caenorhabditis elegans

Autor: H. G. A. M. van Luenen, Ronald H.A. Plasterk, S. R. Wicks, C. J. de Vries
Přispěvatelé: Other departments
Rok vydání: 2000
Předmět:
Zdroj: Developmental biology, 221(2), 295-307. Academic Press Inc.
ISSN: 0012-1606
DOI: 10.1006/dbio.2000.9686
Popis: Forward genetic screens using novel assays of nematode chemotaxis to soluble compounds identified three independent transposon-insertion mutations in the gene encoding the Caenorhabditis elegans dynein heavy chain (DHC) 1b isoform. These disruptions were mapped and cloned using a newly developed PCR-based transposon display. The mutations were demonstrated to be allelic to the che-3 genetic locus. This isoform of dynein shows temporally and spatially restricted expression in ciliated sensory neurons, and mutants show progressive developmental defects of the chemosensory cilia. These results are consistent with a role for this motor protein in the process of intraflagellar transport; DHC 1b acts in concert with a number of other proteins to establish and maintain the structural integrity of the ciliated sensory endings in C. elegans.
Databáze: OpenAIRE