Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts
| Autor: | Tomas Kozak, Sabrina Rueggeberg, Robert Ivanek, Vladimir Koza, Petr Cetkovsky, Sona Pekova, Jiri Schwarz, Xinping Li, Thomas Franz, Jiri Vrba, Stefan Leicht, Michal Dvorak, Miroslav Prucha, Michal Karas |
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| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Myeloid Cellular differentiation Blotting Western Molecular Sequence Data Biology medicine.disease_cause Mice Cell Line Tumor hemic and lymphatic diseases medicine Animals Humans Amino Acid Sequence Cloning Molecular Gene Oligonucleotide Array Sequence Analysis Genetics Mutation Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Genetic Variation Myeloid leukemia Cell Differentiation Exons Hematology medicine.disease Molecular biology body regions Gene expression profiling Leukemia Myeloid Acute Haematopoiesis Leukemia Retroviridae medicine.anatomical_structure fms-Like Tyrosine Kinase 3 Oncology Tandem Repeat Sequences Blast Crisis Cell Division psychological phenomena and processes |
| Zdroj: | Leukemia Research. 33:1409-1416 |
| ISSN: | 0145-2126 |
| Popis: | FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with internal tandem duplications (ITDs) accounting for up to 30% of its mutations. To analyze the impact of individual ITDs on the expression profile of immature myeloid cells, we have established 32D cell lines expressing nine different FLT3/ITDs isolated from AML patients and subjected them to whole genome expression profiling and 2DE/LC/MS proteomics. Our data indicate that in comparison to the controls, FLT3/ITD-positive 32D cells exhibit less mature expression profiles resembling early hematopoietic progenitors. Moreover, our results suggest that there exist biological differences among individual ITD variants. |
| Databáze: | OpenAIRE |
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