A competitive and reversible deactivation approach to catalysis-based quantitative assays
| Autor: | Michael Williams, Matthew P. Tracey, Christopher J. Welch, Kazunori Koide, Xiaodong Bu, Junyong Jo |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
Multidisciplinary 010405 organic chemistry Chemistry Orders of magnitude (temperature) Science General Physics and Astronomy Substrate (chemistry) Nanotechnology General Chemistry 010402 general chemistry 01 natural sciences Combinatorial chemistry Signal Article General Biochemistry Genetics and Molecular Biology 0104 chemical sciences Catalysis Reagent Saturation (chemistry) Order of magnitude |
| Zdroj: | Nature Communications Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016) |
| ISSN: | 2041-1723 |
| Popis: | Catalysis-based signal amplification makes optical assays highly sensitive and widely useful in chemical and biochemical research. However, assays must be fine-tuned to avoid signal saturation, substrate depletion and nonlinear performance. Furthermore, once stopped, such assays cannot be restarted, limiting the dynamic range to two orders of magnitude with respect to analyte concentrations. In addition, abundant analytes are difficult to quantify under catalytic conditions due to rapid signal saturation. Herein, we report an approach in which a catalytic reaction competes with a concomitant inactivation of the catalyst or consumption of a reagent required for signal generation. As such, signal generation proceeds for a limited time, then autonomously and reversibly stalls. In two catalysis-based assays, we demonstrate restarting autonomously stalled reactions, enabling accurate measurement over five orders of magnitude, including analyte levels above substrate concentration. This indicates that the dynamic range of catalysis-based assays can be significantly broadened through competitive and reversible deactivation. Assays for catalytic systems—particularly ones with simple colorimetric readouts—are useful for the rapid evaluation of performance. Here, the authors report an assay based on a concurrent colour-forming reaction working across a wide range that can be stopped to allow measurements and subsequently restarted. |
| Databáze: | OpenAIRE |
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