CMTM5 inhibits renal cancer cell growth through inducing cell-cycle arrest and apoptosis
| Autor: | Shaobin Zheng, Bing Cai, Yunbei Xiao, Yeping Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
renal cell carcinoma Cancer Research tumor suppressor Cell CMTM5 Biology urologic and male genital diseases medicine.disease_cause Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine medicine.diagnostic_test Cell growth Articles Cell cycle 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Carcinogenesis A431 cells |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2017.6350 |
| Popis: | CKLF-like MARVEL transmembrane domain-containing 5 (CMTM5) has been reported to function as a potential tumor suppressor in several human cancers. However, the involvement of CMTM5 in human renal cell carcinoma (RCC) remains unclear. The current study aimed to detect its expression pattern in RCC tissues and cells, and to determine its anti-proliferative functions in this malignancy. The mRNA and protein expression levels of CMTM5 in RCC tissues and cells were detected by reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting. Following the transfection with CMTM5 lentivirus or control lenti-EGFP lentivirus into the RCC cell line ACHN, the viability, migration, apoptosis and cell cycle of these cells were detected by Cell Counting kit-8 assay, Transwell assay and flow cytometry, respectively. Compared with the adjacent non-malignant kidney tissue samples, CMTM5 expression was significantly downregulated in RCC tissues (P |
| Databáze: | OpenAIRE |
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