Force-induced recruitment of cten along keratin network in epithelial cells
| Autor: | Kyle A. Jacobs, Joleen S Cheah, Su Hao Lo, Soichiro Yamada, Volkmar Heinrich |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Mechanotransduction
Image Processing Integrin macromolecular substances Stress Mechanotransduction Cellular Madin Darby Canine Kidney Cells 03 medical and health sciences 0302 clinical medicine Computer-Assisted Dogs Cell Movement Tensins Keratin Image Processing Computer-Assisted Tensin Animals Humans Intermediate filament Cytoskeleton keratin Actin 030304 developmental biology mechanotransduction chemistry.chemical_classification 0303 health sciences Multidisciplinary biology integumentary system Microfilament Proteins tensin simple epithelia Epithelial Cells cytoskeleton Cell Biology Biological Sciences Actin cytoskeleton Mechanical Cell biology chemistry biology.protein Keratins Cellular Stress Mechanical 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, vol 116, iss 40 |
| ISSN: | 1091-6490 |
| Popis: | The cytoskeleton provides structural integrity to cells and serves as a key component in mechanotransduction. Tensins are thought to provide a force-bearing linkage between integrins and the actin cytoskeleton; yet, direct evidence of tensin’s role in mechanotransduction is lacking. We here report that local force application to epithelial cells using a micrometer-sized needle leads to rapid accumulation of cten (tensin 4), but not tensin 1, along a fibrous intracellular network. Surprisingly, cten-positive fibers are not actin fibers; instead, these fibers are keratin intermediate filaments. The dissociation of cten from tension-free keratin fibers depends on the duration of cell stretch, demonstrating that the external force favors maturation of cten−keratin network interactions over time and that keratin fibers retain remarkable structural memory of a cell’s force-bearing state. These results establish the keratin network as an integral part of force-sensing elements recruiting distinct proteins like cten and suggest the existence of a mechanotransduction pathway via keratin network. |
| Databáze: | OpenAIRE |
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