Identification of oligodendrocytes in experimental disease models
| Autor: | Jennifer K. Ness, Mark P. Goldberg, Mario Valentino, Sally R. McIver |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Transgene
Cell Central nervous system Immunocytochemistry Gene Transfer Techniques Biology Immunohistochemistry Oligodendrocyte Disease Models Animal Oligodendroglia Cellular and Molecular Neuroscience medicine.anatomical_structure Neurology In vivo medicine Animals Humans Neuroglia Identification (biology) Neuroscience |
| Zdroj: | Glia. 50:321-328 |
| ISSN: | 1098-1136 0894-1491 |
| DOI: | 10.1002/glia.20206 |
| Popis: | The ability to identify oligodendrocytes in culture, in fixed tissue, and in vivo using unique markers is a requisite step to understanding their responses in any damage, recovery, or developmental process. Their nuclei are readily seen in histological preparations of healthy white and gray matter, and their cell bodies can be reliably identified with a variety of immunocytochemical markers. However, there is little consensus regarding optimal methods to assess oligodendrocyte survival or morphology under experimental injury conditions. We review common approaches for histological and immunocytochemical identification of these cells. Transgenic and viral methods for cell type-selective transfer of genes encoding fluorescent proteins offer promising new approaches for manipulating and visualizing oligodendrocytes in models of health and disease. |
| Databáze: | OpenAIRE |
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