Deciphering the Energy Landscape of the Interaction Uranyl-DCP with Antibodies Using Dynamic Force Spectroscopy

Autor: Michael Odorico, Jean-Luc Pellequer, Jean-Marie Teulon, Pierre Parot
Přispěvatelé: Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service de Biochimie et Toxicologie Nucléaire (SBTN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2008
Předmět:
Biophysical Letters
Stereochemistry
medicine.drug_class
Biophysics
02 engineering and technology
Plasma protein binding
Microscopy
Atomic Force
MESH: Chelating Agents
Monoclonal antibody
MESH: Antibodies
Monoclonal
Metal
03 medical and health sciences
chemistry.chemical_compound
Molecular recognition
Protein Interaction Mapping
medicine
MESH: Phenanthrolines
MESH: Protein Binding
Chelation
MESH: Energy Transfer
Binding site
Chelating Agents
030304 developmental biology
MESH: Microscopy
Atomic Force
0303 health sciences
Binding Sites
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
MESH: Protein Interaction Mapping
Antibodies
Monoclonal
Energy landscape
021001 nanoscience & nanotechnology
Uranyl
Uranium Compounds
Energy Transfer
MESH: Binding Sites
chemistry
visual_art
visual_art.visual_art_medium
MESH: Uranium Compounds
0210 nano-technology
Phenanthrolines
Protein Binding
Zdroj: Biophysical Journal
Biophysical Journal, 2008, 95 (10), pp.L63-L65. ⟨10.1529/biophysj.108.141937⟩
Biophysical Journal, Biophysical Society, 2008, 95 (10), pp.L63-L65. ⟨10.1529/biophysj.108.141937⟩
ISSN: 0006-3495
1542-0086
DOI: 10.1529/biophysj.108.141937
Popis: International audience; Previous studies on molecular recognition of uranyl-DCP (dicarboxy-phenanthroline chelator) compound by two distinct monoclonal antibodies (Mabs U04S and U08S) clearly showed the presence of a biphasic shape in Bell-Evans' plots and an accentuated difference in slopes at the high loading rates. To further explore the basis in the slope difference, we have performed complementary experiments using antibody PHE03S, raised against uranyl-DCP but, presenting a strong cross-reactivity toward the DCP chelator. This work allowed us to obtain a reallocation of the respective contributions of the metal ion itself and that of the chelator. Results led us to propose a 2D schematic model representing two energy barriers observed in the systems Mabs U04S- and U08S-[UO(2)-DCP] where the outer barrier characterizes the interaction between UO(2) and Mab whereas the inner barrier characterizes the interaction between DCP and Mab. Using dynamic force spectroscopy, it is thus possible to dissect molecular interactions during the unbinding between proteins and ligands.
Databáze: OpenAIRE
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