SUBCELLULAR DISTRIBUTION OF AROMATIC AMINO ACID TRANSAMINASES IN RAT BRAIN
| Autor: | M. Civen, T. Semba |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Male
Serotonin Tryptophan Transaminase Dopamine Tyrosine Transaminase Centrifugation Benzoates Biochemistry Synaptic vesicle Transaminase 5-Hydroxytryptophan Cellular and Molecular Neuroscience chemistry.chemical_compound Microsomes Aromatic amino acids medicine Animals Amines Transaminases chemistry.chemical_classification Chemistry Tryptophan Brain Dihydroxyphenylalanine Mitochondria Rats Enzyme Spectrophotometry Microsome medicine.drug |
| Zdroj: | Journal of Neurochemistry. 17:795-800 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1970.tb03351.x |
| Popis: | — The subcellular distributions of tyrosine transaminase, DOPA transaminase, tryptophan transaminase and 5-hydroxytryptophan (5-HTP) transaminase were studied in rat brain. 1 For all of these transaminases 60-81 per cent of the total activities were found in the crude mitochondrial fraction. Tyrosine transaminase was the most active enzyme. 2 Tyrosine transaminase and DOPA transaminase had very similar distributions in all fractions, but the distribution of tryptophan transaminase and 5-HTP transaminase differed in the microsomal (Mic) and synaptic vesicle (M2) fractions. Only 5-HTP transaminase was highly concentrated in the M2 fraction. 3 DOPA transaminase was inhibited by dopamine and 5-HT, but these compounds had no effect on 5-HTP transaminase. Both enzymes were completely inhibited by m-hydroxybenzoyloxyamine. |
| Databáze: | OpenAIRE |
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