Adenosine and adenosine-5′-monophosphate ingestion ameliorates abnormal glucose metabolism in mice fed a high-fat diet
| Autor: | Takuya Koseki, Hitoshi Shirakawa, Ardiansyah, Tomoko Goto, Yuto Inagawa, Michio Komai, Ikuo Ikeda, Afifah Zahra Agista |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine Adenosine monophosphate medicine.medical_specialty Adenosine AMP-Activated Protein Kinases Carbohydrate metabolism Diet High-Fat Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Lipid oxidation Internal medicine medicine Animals Humans Hypoglycemic Agents Insulin Ingestion Obesity Phosphorylation Muscle Skeletal Glucose metabolism Chemistry Insulin tolerance test AMPK General Medicine lcsh:Other systems of medicine Glucose Tolerance Test medicine.disease lcsh:RZ201-999 Adenosine Monophosphate Mice Inbred C57BL 030104 developmental biology Endocrinology High-fat diet Complementary and alternative medicine Adenosine-5′-monophosphate 030217 neurology & neurosurgery Research Article medicine.drug |
| Zdroj: | BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-8 (2018) BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-018-2367-6 |
| Popis: | Background We have previously reported that ingestion of adenosine (ADN) and adenosine-5′-monophosphate (AMP) improves abnormal glucose metabolism in the stroke-prone spontaneously hypertensive rat model of non-obesity-associated insulin resistance. In this study, we investigated the effect of ADN and AMP ingestion on glucose metabolism in mice with high-fat diet-induced obesity. Methods Seven-week-old C57BL/6 J mice were administered distilled water (as a control), 10 mg/L ADN, or 13 mg/L AMP via their drinking water for 14 or 25 weeks, during which they were fed a high-fat diet. Oral glucose tolerance test (OGTT) was conducted on 21-week-old mice fasted for 16 h. Insulin tolerance test (ITT) was performed on 22-week-old mice fasted for 3 h. Blood and muscle were collected for further analysis of serum parameters, gene and protein expression levels, respectively. Results Glucose metabolism in the ADN and AMP groups was significantly improved compared with the control. OGTT and ITT showed that ADN and AMP groups lower than control group. Furthermore, phosphorylation of AMP-activated protein kinase (AMPK) and mRNA levels of genes involved in lipid oxidation were enhanced in the skeletal muscle of ADN- and AMP-treated mice. Conclusion These results indicate that ingestion of ADN or AMP induces activation of AMPK in skeletal muscle and mitigates insulin resistance in mice with high-fat diet-induced diabetes. Electronic supplementary material The online version of this article (10.1186/s12906-018-2367-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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