Development of an injectable PHBV microparticles-GG hydrogel hybrid system for regenerative medicine
| Autor: | Daniela Peneda Pacheco, Rui L. Reis, Alexandra P. Marques, Vitor M. Correlo, Maria Helena Amaral |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Polyesters
Pharmaceutical Science Context (language use) Regenerative medicine Dexamethasone Injections Polyhydroxybutyrate chemistry.chemical_compound Drug Delivery Systems Polysaccharides Gellan gum Injectable hydrogel Microparticulate systems Polyhydroxybutyrate-co-hydroxyvalerate Drug Liberation Hydrogels Polysaccharides Bacterial Regenerative Medicine Serum Albumin Bovine 3003 Bovine serum albumin Serum Albumin Science & Technology biology Bacterial Bovine Gellan Gum 3. Good health chemistry Chemical engineering Drug delivery Self-healing hydrogels biology.protein |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP International Journal of Pharmaceutics |
| Popis: | Uncontrollable displacements that greatly affect the concentration of active agents at the target tissues are among a major limitation of the use of microparticulate drug delivery systems (DDS). Under this context a biphasic injectable DDS combining poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles (MPs) and a gellan gum (GG) injectable hydrogel is herein proposed for the localized delivery and long-term retention of MPs carrying hydrophilic and hydrophobic model active agents. A double emulsion-solvent evaporation method was adopted to develop the PHBV MPs, carrying bovine serum albumin (BSA) or dexamethasone (Dex) as hydrophilic and hydrophobic active agents’ models, respectively. Moreover, this method was modified, together with the properties of the hydrogel to tailor the delivery profile of the active agents. Variations of the composition of the organic phase during the process allowed tuning surface topography, particle size distribution and core porosity of the PHBV MPs and, thus, the in vitro release profile of Dex but not of BSA. Besides, after embedding hydrogels of higher GG concentration led to a slower and more sustained release of both active agents, independently of the processing conditions of the microparticulate system. The authors would like to acknowledge the Project RL1 - ABMR - NORTE-01-0124-FEDER-000016 co-financed by North Portugal Regional Operational Programme (ON.2 - O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF). This work was partially supported by European Research Council grant agreement ERC-2012-ADG 20120216-321266 for project ComplexiTE. |
| Databáze: | OpenAIRE |
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