Fast and Efficient Neural Conversion of Human Hematopoietic Cells
| Autor: | Kai-Christian Sonntag, Marco Straccia, Cristina Bruzos-Cidón, Amaia Zubiarrain, Lorea Zabaleta, Luisa Ugedo, María Torrecilla, Xonia Carvajal-Vergara, Josep M. Canals, Amaia Sousa, Nerea Vazquez, Pablo Menendez, Julio Castaño, Alessandra Giorgetti, Rosario Sanchez-Pernaute |
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| Přispěvatelé: | Universitat de Barcelona |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Somatic cell
Gene Expression Biochemistry in-vivo dopaminergic-neurons Membrane Potentials Immunophenotyping definer factors Cell physiology Xarxes neuronals (Neurobiologia) AC133 Antigen Neural networks (Neurobiology) lcsh:QH301-705.5 Cells Cultured Cellular Senescence Neurons Fisiologia cel·lular adult human fibroblasts lcsh:R5-920 Leucòcits funtional-neurons progenitor cells Fetal Blood Cell biology Neuroepithelial cell Haematopoiesis Phenotype Cord blood Cell Transdifferentiation cord blood lcsh:Medicine (General) Cell aging Resource Biology SOX2 Antigens CD Genetics Humans Leucocytes Cell Proliferation Glycoproteins induced neuronal cells Gene Expression Profiling pluripotent stem-cells mouse fibroblasts Cell Biology lcsh:Biology (General) Immunology Leukocytes Mononuclear Peptides Developmental Biology |
| Zdroj: | Stem Cell Reports, Vol 3, Iss 6, Pp 1118-1131 (2014) Recercat. Dipósit de la Recerca de Catalunya instname Addi. Archivo Digital para la Docencia y la Investigación Stem Cell Reports Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | Summary Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for generating induced neural cells (iNCs) directly from human hematopoietic cells using Sendai virus. Upon SOX2 and c-MYC expression, CD133-positive cord blood cells rapidly adopt a neuroepithelial morphology and exhibit high expansion capacity. Under defined neurogenic culture conditions, they express mature neuronal markers and fire spontaneous action potentials that can be modulated with neurotransmitters. SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs. However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation. Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency. Graphical Abstract Highlights • Expandable iNCs are generated from CB cells by SeV at high efficiency • CB-iNCs differentiate into functional neurons in vitro • CB stem/progenitor cells are more amenable to neural conversion than PB-MNCs Giorgetti, Sanchez-Pernaute and colleagues report a fast and efficient approach for generating functional induced neurons from both cord blood and peripheral blood cells using SOX2 and c-MYC Sendai virus. However, the reprogramming of adult peripheral blood cells is less efficient. This study provides additional evidence that the origin and the nature of target cells may impact the outcome of the conversion process. |
| Databáze: | OpenAIRE |
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