Biological properties of rabbit antibodies to a surface antigen of Rickettsia rickettsii
| Autor: | M R Johnston, R E Mann, R L Anacker, Robert N. Philip, C J Nauck, E Casper, W J Todd |
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| Rok vydání: | 1983 |
| Předmět: |
Male
Immunoelectron microscopy Rocky Mountain spotted fever Guinea Pigs Immunology Rickettsia rickettsii Microbiology Antigen-Antibody Reactions Guinea pig Mice Antigen medicine Animals Serotyping Rocky Mountain Spotted Fever Antiserum biology Immune Sera Immunization Passive biology.organism_classification medicine.disease Antibodies Bacterial Virology Spotted fever Infectious Diseases Antigens Surface biology.protein bacteria Parasitology Rabbits Antibody Immunoelectrophoresis Two-Dimensional Research Article |
| Zdroj: | Infection and Immunity. 40:292-298 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.40.1.292-298.1983 |
| Popis: | Because of the potential significance of external components of the obligate intracellular parasite Rickettsia rickettsii in host-parasite interactions, we have begun the first phase of a study to isolate and characterize surface antigens of this organism. An antiserum to a rickettsial surface component was obtained from rabbits inoculated with immune precipitates prepared by crossed immunoelectrophoresis of Triton X-100 extracts of R. rickettsii strain R. This antiserum (i) protected guinea pigs inoculated with 10,000 guinea pig 50% infectious doses of R. rickettsii against fever, (ii) prevented death of mice challenged with 2 50% lethal doses of R. rickettsii, and (iii) reacted in the microimmunofluorescence test with 9 of 13 spotted fever group serotypes tested. The location of this antigen on the rickettsial surface was demonstrated by immunoelectron microscopy with ferritin-labeled antibodies. |
| Databáze: | OpenAIRE |
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