The mechanism analysis using PI3K/AKT pathway for the effects of levocarnitine in the treatment of spermatogenic dysfunction
| Autor: | Bin Wang, Junlong Feng, Sheng Deng, Fanchao Meng, Hengheng Dai, Haisong Li, Bing-Hao Bao, Jisheng Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Spermatozoon Chemistry Urology Motility General Medicine Sperm Rats Levocarnitine Andrology Phosphatidylinositol 3-Kinases Endocrinology medicine.anatomical_structure In vivo Apoptosis Carnitine Sperm Motility medicine Animals Humans Spermatogenesis Proto-Oncogene Proteins c-akt PI3K/AKT/mTOR pathway Sperm motility |
| Zdroj: | Andrologia. 54 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1111/and.14290 |
| Popis: | LEV improves the percentage of forward-motion spermatozoon and total sperm motility in patients with oligozoospermia or asthenospermia in clinical settings. However, the mechanism of action of levocarnitine (LEV) in the treatment of spermatogenic dysfunction was unclear. Based on in vitro and in vivo experiments, we used Glucosides of Tripterygium wilfordii Hook F (GTW) to construct a cell model (using spermatogenic GC-1 spg cells) and an animal model (using rats) of spermatogenic dysfunction. LEV and LY294002 (a PI3K pathway inhibitor) were then administered. By assessing apoptosis and sperm quality and motility, the underlying mechanism was explored. We found that GTW induced spermatogenic dysfunction, and LEV ameliorated the GTW-induced spermatogenic dysfunction. LEV inhibited GC-1 spg cell apoptosis and improved the sperm count and percentages of PR (forward motion) + NP (non-forward motion) (p |
| Databáze: | OpenAIRE |
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