Effects of sex hormone binding globulin (SHBG) on human prostatic carcinoma
| Autor: | Kristine M. Wiren, Rita C. Hoop, Richard C. Ostenson, William Rosner, D J Hryb, Steven M. Loop, Stephen R. Plymate |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry Gene Expression In Vitro Techniques Biochemistry Endocrinology Sex hormone-binding globulin Cell surface receptor Internal medicine Sex Hormone-Binding Globulin polycyclic compounds medicine Tumor Cells Cultured Humans Testosterone RNA Messenger Receptor Autocrine signalling Molecular Biology reproductive and urinary physiology biology Cell growth Cell Cycle Cell Membrane Albumin Prostatic Neoplasms Biological Transport Dihydrotestosterone Cell Biology Blotting Northern Cell culture biology.protein Molecular Medicine hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 40(4-6) |
| ISSN: | 0960-0760 |
| Popis: | The purpose of this study was to determine what effects sex hormone binding globulin (SHBG) might have on the growth and steroid content of human prostate carcinoma. Two human prostate carcinoma cell lines were used for this study, ALVA-41 and ALVA-101. The first part of the study was to determine the effect of SHBG or albumin on the uptake of [3H]DHT in the cells. In this experiment both SHBG and albumin inhibits the uptake of [3H]DHT into each of the cell lines when studied in vitro. The degree of inhibition was dependent on the binding capacity of the protein. When [3H]thymidine uptake was measured in each of the cell lines following either the addition of SHBG or albumin to the culture media, an increase in uptake and presumably DNA synthesis was noted in the ALVA-41 and ALVA-101 cells for SHBG additions but not for albumin. Further, this stimulation was increased when testosterone was added to the media, however, [3H]thymidine uptake was decreased by high concentrations of dihydrotestosterone (DHT) or if the SHBG was saturated with DHT prior to being added to the media. The cells also demonstrate high affinity cell membrane receptors for SHBG. Finally, using a 3', 550 bp cDNA or SHBG, 1.9 and 2.8 kb mRNAs were detected on Northern analysis of the ALVA-101 and ALVA-41 cells. These data indicate SHBG can inhibit uptake of steroids into the prostate, but also it may act as a stimulus for growth through a SHBG cell surface receptor. In addition, the growth effect may be through an autocrine effect from SHBG or a SHBG-related peptide. |
| Databáze: | OpenAIRE |
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